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However, the commercially available LH immunoassays are developed and validated for human blood samples but not urine so that LH assays intended for use with urine samples need thorough validation. Therefore, the present study evaluated the measurement of urinary LH immunoreactivity using previously validated immunofluorometric (IF) and immunochemiluminometric (ICL) LH assays after prolonged frozen storage. LH was measured in serial urine samples following administration of a single injection of one of two doses of recombinant human chorionic hormone (rhCG) with assays run at the end of study (2008) and again after four years of frozen (\u201020\u2009\u00b0C) storage where samples were stored without adding preservatives. The ICL assay showed quantitatively reproducible LH measurements after prolonged \u201020\u2009\u00b0C storage. However, the IF immunoassay gave consistently lower LH levels relative to ICL (2008) with a further proportionate reduction after four years of sample storage (2012). Yet, both the assays displayed similar patterns of the time\u2010course of urine LH measurement both before and after four years of frozen storage. In conclusion, we found that both immunoassays are suitable for urinary LH measurements with ICL assay being more robust for quantitative urinary LH measurement such as for anti\u2010doping purposes, whereas the IF could be applicable for research studies where urine LH levels are compared within\u2010study but not in absolute terms. Copyright \u00a9 2013 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/dta.1481","type":"journal-article","created":{"date-parts":[[2013,4,21]],"date-time":"2013-04-21T23:00:47Z","timestamp":1366585247000},"page":"336-341","source":"Crossref","is-referenced-by-count":14,"title":["Immunoreactive LH in long\u2010term frozen human urine samples"],"prefix":"10.1002","volume":"6","author":[{"given":"Gurmeet Kaur Surindar","family":"Singh","sequence":"first","affiliation":[{"name":"Andrology Laboratory, ANZAC Research Institute  Sydney NSW 2139 Australia"},{"name":"Faculty of Pharmacy Universiti Teknologi MARA  Bandar Puncak Alam Selangor 42300 Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark","family":"Jimenez","sequence":"additional","affiliation":[{"name":"Andrology Laboratory, ANZAC Research Institute  Sydney NSW 2139 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ron","family":"Newman","sequence":"additional","affiliation":[{"name":"Andrology Laboratory, ANZAC Research Institute  Sydney NSW 2139 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David J.","family":"Handelsman","sequence":"additional","affiliation":[{"name":"Andrology Laboratory, ANZAC Research Institute  Sydney NSW 2139 Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,4,21]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1258\/000456306777695582"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-197201000-00003"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/1687-9856-2012-10"},{"key":"e_1_2_5_5_1","first-page":"17","article-title":"Measuring endocrine profiles of women in field studies","volume":"25","author":"Kesner J. 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