{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T11:54:00Z","timestamp":1783857240769,"version":"3.55.0"},"reference-count":23,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,6,17]],"date-time":"2013-06-17T00:00:00Z","timestamp":1371427200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:p>Semen Strychni, a known toxic drug in Chinese pharmacopoeia, is notable for its therapeutic effects on local muscle and joint pain. However, oral administration can be risky. Topically administered drugs accumulate in the topical muscles and knee joints without any major increase in plasma levels; only non\u2010protein\u2010bound drugs in the biological fluids of target tissues are effective for therapeutic effects. A sensitive and rapid ultra performance liquid chromatography \u2010 mass spectrometry (UPLC\u2010MS) method coupled with a microdialysis technique was developed to determine the non\u2010protein\u2010bound strychnine (Str) and brucine (Bru) in rabbit muscle and synovial fluid microdialysate. The UPLC separation was carried out using a 1.7\u03bcm BEH C18 column (50 mm\u2009\u00d7\u20092.1 mm) with a mobile phase consisting of methanol: water (29.5:70.5, <jats:italic>v<\/jats:italic>\/<jats:italic>v<\/jats:italic>) with 0.1% formic acid and 20 mM ammonium acetate in water. The method was validated at concentrations ranging from 0.58 ng\/ml to 467.20 ng\/ml for Str and from 0.42 ng\/ml to 422.40 ng\/ml for Bru. Intra\u2010day and inter\u2010day accuracy ranged from 99.1% to 103.2% for Str and from 95.8% to 108.8% for Bru with intra\u2010day and inter\u2010day precision within 9.7%. The proposed method was successfully applied to determine non\u2010protein\u2010bound Str and Bru, and the analysates concentration remained stable in rabbit muscle and synovial fluid after topical application of total <jats:italic>Strychnos<\/jats:italic> alkaloid patches, which indicated that total <jats:italic>Strychnos<\/jats:italic> alkaloid patches could substitute for the traditional oral administration of Semen Strychni. Copyright \u00a9 2013 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/dta.1493","type":"journal-article","created":{"date-parts":[[2013,6,17]],"date-time":"2013-06-17T02:58:16Z","timestamp":1371437896000},"page":"357-362","source":"Crossref","is-referenced-by-count":9,"title":["Dynamic detection of non\u2010protein\u2010bound strychnine and brucine in rabbit muscle and synovial fluid after topical application of total <i>Strychnos<\/i> alkaloid patches"],"prefix":"10.1002","volume":"6","author":[{"given":"Huaibo","family":"Tang","sequence":"first","affiliation":[{"name":"Institute of Clinical Pharmacy the Second Xiangya Hospital, Central South University  Changsha 410011 China"},{"name":"Department of Pharmacy, Chemistry College Xiangtan University  Xiangtan 411105 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miao","family":"Yan","sequence":"additional","affiliation":[{"name":"Institute of Clinical Pharmacy the Second Xiangya Hospital, Central South University  Changsha 410011 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huande","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Clinical Pharmacy the Second Xiangya Hospital, Central South University  Changsha 410011 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianrong","family":"Xun","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Chemistry College Xiangtan University  Xiangtan 411105 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Deng","sequence":"additional","affiliation":[{"name":"Institute of Clinical Pharmacy the Second Xiangya Hospital, Central South University  Changsha 410011 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeye","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Chemistry College Xiangtan University  Xiangtan 411105 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Long","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Chemistry College Xiangtan University  Xiangtan 411105 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,6,17]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"47","volume-title":"Pharmacopoeia of Peoples Republic of China","author":"National Commission of Chinese Pharmacopoeia","year":"2010"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-8741(03)00224-1"},{"key":"e_1_2_6_4_1","first-page":"81","volume-title":"Modern Chinese Material Medica","author":"Xiao P.G.","year":"2002"},{"key":"e_1_2_6_5_1","first-page":"458","volume-title":"Poison Detecting Diagnosis and Treatment of Acute Poisoning","author":"Li H.D.","year":"2000"},{"key":"e_1_2_6_6_1","first-page":"747","article-title":"Quantitative determination of strychnine and brucine in seeds of commercial Semen Nux\u2010vomica by RP\u2010H PLC","volume":"31","author":"Gu Z.P.","year":"1996","journal-title":"Chin. 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