{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T06:02:16Z","timestamp":1791093736831,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"9-10","license":[{"start":{"date-parts":[[2013,6,10]],"date-time":"2013-06-10T00:00:00Z","timestamp":1370822400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:p>The diester\u2010diterpene alkaloid aconitine was quantified by liquid chromatography\u2010tandem mass spectrometry in post\u2010mortem specimens of three cases where suicidal ingestion of <jats:italic>Aconitum napellus<\/jats:italic> L. (\u2018monkshood\u2019) was supposed. In an attempt at rationalization, sample preparation and chromatographic conditions of plasma\/serum drug analysis routine were utilized. Linearity was established from 0.5 to 20\u2009\u00b5g\u2009L<jats:sup>\u20101<\/jats:sup> using newborn calf serum (NCS) as a surrogate calibration matrix for all sample types and mesaconitine as an internal standard. Validation (selectivity, sensitivity, precision, accuracy, recovery of the extraction procedure, matrix effect, processed sample stability) confirmed the applicability of the analytical method to various post\u2010mortem matrices. Internal standard selection was based on multi\u2010matrix process efficiency data. In human post\u2010mortem peripheral blood a lower limit of quantification of 0.51\u2009\u00b5g\u2009L<jats:sup>\u20101<\/jats:sup> and a limit of detection of 0.13\u2009\u00b5g\u2009L<jats:sup>\u20101<\/jats:sup> were accomplished (0.1\u2009ml sample aliquots). Aconitine was degraded to a large extent in different sample types when being stored at +20\u2009\u00b0C for 30\u2009days, while at \u201020\u2009\u00b0C and for some matrices also at +4\u2009\u00b0C no appreciable degradation occurred. Aconitine concentrations in real samples were 10.3\u201317.9\u2009\u00b5g\u2009L<jats:sup>\u20101<\/jats:sup> (peripheral blood, <jats:italic>n<\/jats:italic>\u2009=\u20093), 14.9\u201087.9\u2009\u00b5g\u2009L<jats:sup>\u20101<\/jats:sup> (heart blood, <jats:italic>n<\/jats:italic>\u2009=\u20093), 317\u2010481\u2009\u00b5g\u2009L<jats:sup>\u20101<\/jats:sup> (urine, <jats:italic>n<\/jats:italic>\u2009=\u20092), 609\u20134040\u2009\u00b5g\u2009L<jats:sup>\u20101<\/jats:sup> (stomach content, <jats:italic>n<\/jats:italic>\u2009=\u20093), 139\u2010240\u2009\u00b5g\u2009L<jats:sup>\u20101<\/jats:sup> (bile, <jats:italic>n<\/jats:italic>\u2009=\u20092), 8.4\u2009\u00b5g\u2009L<jats:sup>\u20101<\/jats:sup> (vitreous humor, <jats:italic>n<\/jats:italic>\u2009=\u20091), 54.7\u2009\u00b5g\u2009L<jats:sup>\u20101<\/jats:sup> (pericardial fluid, <jats:italic>n<\/jats:italic>\u2009=\u20091), 492\u2009\u00b5g\u2009kg<jats:sup>\u20101<\/jats:sup> (liver, <jats:italic>n<\/jats:italic>\u2009=\u20091), 15.2\u201319.7\u2009mg\u2009L<jats:sup>\u20101<\/jats:sup> (unknown liquids secured onsite, <jats:italic>n<\/jats:italic>\u2009=\u20093). Together with concomitant circumstances the analytical data provided compelling evidence for acute <jats:italic>Aconitum<\/jats:italic> poisoning as being the cause of death. Copyright \u00a9 2013 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/dta.1501","type":"journal-article","created":{"date-parts":[[2013,6,10]],"date-time":"2013-06-10T02:33:13Z","timestamp":1370831593000},"page":"753-762","source":"Crossref","is-referenced-by-count":22,"title":["Quantification of aconitine in post\u2010mortem specimens by validated liquid chromatography\u2010tandem mass spectrometry method: Three case reports on fatal \u2018monkshood\u2019 poisoning"],"prefix":"10.1002","volume":"5","author":[{"given":"Wolfgang","family":"Bicker","sequence":"first","affiliation":[{"name":"FTC\u2010Forensic\u2010Toxicological Laboratory Ltd.  Gaudenzdorfer Guertel 43\u201045 1120 Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabio","family":"Monticelli","sequence":"additional","affiliation":[{"name":"Institute of Forensic Medicine University of Salzburg  Ignaz\u2010Harrer\u2010Strasse 79 5020 Salzburg Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Bauer","sequence":"additional","affiliation":[{"name":"Institute of Forensic Medicine Ludwig\u2010Maximilians\u2010University Munich  Nussbaumstrasse 26 80336 Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriele","family":"Roider","sequence":"additional","affiliation":[{"name":"Institute of Forensic Medicine Ludwig\u2010Maximilians\u2010University Munich  Nussbaumstrasse 26 80336 Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Keller","sequence":"additional","affiliation":[{"name":"Institute of Forensic Medicine University of Salzburg  Ignaz\u2010Harrer\u2010Strasse 79 5020 Salzburg Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,6,10]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/22.4.336"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/24.5.348"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1355-0306(02)71807-8"},{"key":"e_1_2_7_5_1","unstructured":"H.Niinuma H.Aoki T.Suzuki M.Shibata Y.Moriai T.Suzuki et al.Two survival cases of severe aconite poisoning by percutaneous cardiopulmonary support system and cardiopulmonary bypass for fatal arrhythmia: A case report.The Internet Journal of Emergency and Intensive Care Medicine. 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