{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T12:02:06Z","timestamp":1780056126517,"version":"3.54.0"},"reference-count":33,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2014,3,10]],"date-time":"2014-03-10T00:00:00Z","timestamp":1394409600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001824","name":"Czech Science Foundation","doi-asserted-by":"crossref","award":["P502\/10\/0217"],"award-info":[{"award-number":["P502\/10\/0217"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2014,10]]},"abstract":"<jats:p>Monepantel (MOP) is a new anthelmintic drug intended for the treatment and control of gastrointestinal roundworms (nematodes) infection and associated disease in sheep. The aim of our study was to find out metabolic pathways of MOP in sheep <jats:italic>in vivo<\/jats:italic> and in its parasite <jats:italic>Haemonchus contortus ex vivo<\/jats:italic>. MOP biotransformation in two <jats:italic>H<\/jats:italic>. <jats:italic>contortus<\/jats:italic> strains with different sensitivity to anthelmintics was also compared. Ultra high\u2010performance liquid chromatography with tandem mass spectrometry (UHPLC\u2010MS\/MS) technique is used for the identification of MOP metabolites in ovine urine, faeces, and nematodes. MOP biotransformation study in sheep <jats:italic>in vivo<\/jats:italic> led to the identification of 13 MOP metabolites; 7 of them have not been described previously in <jats:italic>in vitro<\/jats:italic> study. The study of MOP biotransformation in <jats:italic>H<\/jats:italic>. <jats:italic>contorus ex vivo<\/jats:italic> reveals four MOP metabolites. The nitrile hydrolysis as a new biotransformation pathway in helminths <jats:italic>ex vivo<\/jats:italic> is reported here for the first time. Unlike sheep, <jats:italic>H<\/jats:italic>. <jats:italic>contorus<\/jats:italic> nematodes are not able to metabolize MOP via phase II biotransformation. Nematodes of resistant White river (WR) strain form more types of MOP metabolites than nematodes of sensitive inbred susceptible Edinburgh (ISE) strain. Based on obtained results, schemes of metabolic pathways of MOP in sheep and nematodes are proposed. Copyright \u00a9 2014 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/dta.1630","type":"journal-article","created":{"date-parts":[[2014,3,10]],"date-time":"2014-03-10T15:37:56Z","timestamp":1394465876000},"page":"1055-1062","source":"Crossref","is-referenced-by-count":24,"title":["Metabolic pathways of anthelmintic drug monepantel in sheep and in its parasite (<i>Haemonchus contortus<\/i>)"],"prefix":"10.1002","volume":"6","author":[{"given":"Lucie","family":"Stuchl\u00edkov\u00e1","sequence":"first","affiliation":[{"name":"Department of Biochemical Sciences, Faculty of Pharmacy in Hradec Kr\u00e1lov\u00e9 Charles University in Prague  Heyrovsk\u00e9ho 1203 500 05 Hradec Kr\u00e1lov\u00e9 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Jir\u00e1sko","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry, Faculty of Chemical Technology University of Pardubice  Studentsk\u00e1 573 532 10 Pardubice Czech 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