{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T16:52:51Z","timestamp":1775148771159,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,7,31]],"date-time":"2014-07-31T00:00:00Z","timestamp":1406764800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:p>Therapeutic drug monitoring of antipsychotics is important in optimizing individual therapy. In psychiatric populations, classical venous blood sampling is experienced as frightening. Interest in alternative techniques, like dried blood spots (DBS), has consequently increased. A fast and easy to perform DBS method for quantification of 16 antipsychotics (amisulpride, aripiprazole, asenapine, bromperidol, clozapine, haloperidol, iloperidone, levosulpiride, lurasidone, olanzapine, paliperidone, pipamperone, quetiapine, risperidone, sertindole and zuclopenthixol) and 8 metabolites was developed. DBS were prepared using 25\u2009\u03bcL of whole blood and extraction of complete spots was performed using methanol: methyl\u2010t\u2010butyl\u2010ether (4:1). After evaporation, the extract was reconstituted in the mobile phase and 10\u2009\u03bcL were injected on an ultra\u2010high performance liquid chromatography\u2010tandem mass spectrometry (UHPLC\u2010MS\/MS). Separation using a C18 column and gradient elution with a flow rate of 0.5\u2009mL\/min resulted in a 6\u2010min run\u2010time. Ionization was performed in positive mode and a dynamic MRM method was applied. Median recovery was 66.4 % (range 28.7\u201084.5%). Accuracy was within the acceptance criteria, except for pipamperone (LLOQ and low concentration) and lurasidone (low concentration). Imprecision was only aberrant for lurasidone at low and medium concentration. All compounds were stable during 1\u2009month at room temperature, 4\u2009\u00b0C and \u221218\u2009\u00b0C. Lurasidone was unstable when the extract was stored for 12\u2009h on the autosampler. Absolute matrix effects (ME) (median 66.1%) were compensated by the use of deuterated IS (median 98.8%). The DBS method was successfully applied on 25\u2010\u03bcL capillary DBS from patients and proved to be a reliable alternative for quantification of all antipsychotics except for olanzapine and N\u2010desmethylolanzapine. 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