{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T00:11:47Z","timestamp":1760487107885,"version":"build-2065373602"},"reference-count":16,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,5,14]],"date-time":"2015-05-14T00:00:00Z","timestamp":1431561600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:p>Piperaquine\u2010dihydroartemisinin combination is the latest addition to the repertoire of ACTs recommended by the World Health Organization (WHO) for treatment of falciparum malaria. Due to the increasing resistance to artemisinin derivatives, CSIR\u2010CDRI has developed a prospective short acting, trioxane antimalarial derivative, CDRI 97\u201078. In the present study, a liquid chromatography\u2010electrospray ionization\u2010tandem mass spectrometry (LC\u2013ESI\u2010MS\/MS) method for the simultaneous quantification of piperaquine (PPQ) and 97\u201063, the active metabolite of CDRI 97\u201078 found <jats:italic>in vivo<\/jats:italic>, was developed and validated in 100\u2009\u03bcL rat plasma using halofantrine as internal standard. PPQ and 97\u201063 were separated using acetonitrile:methanol (50:50, <jats:italic>v<\/jats:italic>\/<jats:italic>v<\/jats:italic>) and ammonium formate buffer (10\u2009mM, pH\u20094.5) in the ratio of 95:5(<jats:italic>v<\/jats:italic>\/<jats:italic>v<\/jats:italic>) as mobile phase under isocratic conditions at a flow rate of 0.65\u2009mL\/min on Waters Atlantis C<jats:sub>18<\/jats:sub> (4.6\u2009\u00d7\u200950\u2009mm, 5.0\u2009\u00b5m) column. The extraction recoveries of PPQ and 97\u201063 ranged from 90.58 to 105.48%, while for the internal standard, it was 94.27%. The method was accurate and precise in the linearity range 3.9\u2013250\u2009ng\/mL for both the analytes, with a correlation coefficient (r) of\u2009\u2265\u20090.998. The intra\u2010 and inter\u2010day assay precision ranged from 2.91 to 8.45% and; intra\u2010 and inter\u2010day assay accuracy was between 92.50 and 110.20% for both the analytes. The method was successfully applied to study the effect of oral co\u2010administration of PPQ on the pharmacokinetics of CDRI 97\u201078 in <jats:italic>Sprague\u2010dawley<\/jats:italic> rats and vice versa. The co\u2010administration of CDRI 97\u201078 caused significant decrease in AUC<jats:sub>0\u2013\u221e<\/jats:sub> of PPQ from 31.52\u2009\u00b1\u20092.68 to 14.84\u2009\u00b1\u20094.33\u2009h*\u00b5g\/mL. However, co\u2010administration of PPQ did not have any significant effect on the pharmacokinetics of CDRI 97\u201078. Copyright \u00a9 2015 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/dta.1807","type":"journal-article","created":{"date-parts":[[2015,5,14]],"date-time":"2015-05-14T22:51:25Z","timestamp":1431643885000},"page":"221-227","source":"Crossref","is-referenced-by-count":5,"title":["Development and validation of an LC\u2010MS\/MS method for simultaneous determination of piperaquine and 97\u201063, the active metabolite of CDRI 97\u201078, in rat plasma and its application in interaction study"],"prefix":"10.1002","volume":"8","author":[{"given":"Muhammad","family":"Wahajuddin","sequence":"first","affiliation":[{"name":"Pharmacokinetics and Metabolism Division CSIR\u2010 Central Drug Research Institute  Lucknow Uttar Pradesh India"},{"name":"Academy of Scientific and Industrial Research  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheelendra Pratap","family":"Singh","sequence":"additional","affiliation":[{"name":"Analytical Chemistry Division CSIR\u2010Indian Institute of Toxicology Research  Lucknow Uttar Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isha","family":"Taneja","sequence":"additional","affiliation":[{"name":"Pharmacokinetics and Metabolism Division CSIR\u2010 Central Drug Research Institute  Lucknow Uttar Pradesh India"},{"name":"Academy of Scientific and Industrial Research  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kanumuri Siva Rama","family":"Raju","sequence":"additional","affiliation":[{"name":"Pharmacokinetics and Metabolism Division CSIR\u2010 Central Drug Research Institute  Lucknow Uttar Pradesh India"},{"name":"Academy of Scientific and Industrial Research  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiaur Rahman","family":"Gayen","sequence":"additional","affiliation":[{"name":"Pharmacokinetics and Metabolism Division CSIR\u2010 Central Drug Research Institute  Lucknow Uttar Pradesh India"},{"name":"Academy of Scientific and Industrial Research  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hefazat Hussain","family":"Siddiqui","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy Integral University  Lucknow India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shio Kumar","family":"Singh","sequence":"additional","affiliation":[{"name":"Pharmacokinetics and Metabolism Division CSIR\u2010 Central Drug Research Institute  Lucknow Uttar Pradesh India"},{"name":"Academy of Scientific and Industrial Research  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,5,14]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-200565010-00004"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jps.21226"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/clpt.2011.254"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2125.2003.02004.x"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0808859"},{"key":"e_1_2_5_7_1","unstructured":"World Health Organisation.World Malaria Report 2013. World Health Organization Geneva 2013."},{"volume-title":"Spiro\u20101, 2, 4\u2010trioxanes, US Patent 7495025 B2","year":"2009","author":"Singh C.","key":"e_1_2_5_8_1"},{"volume-title":"Substituted 1, 2, 4\u2010trioxanes as antimalarial agents and a process of producing the substituted 1, 2, 4\u2010trioxanes, in US Patent 6316493 B1","year":"2001","author":"Singh C.","key":"e_1_2_5_9_1"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0031-1296177"},{"key":"e_1_2_5_11_1","first-page":"372521","article-title":"Single ascending dose safety and pharmacokinetics of CDRI\u201097\/78: First\u2010in\u2010human study of a novel antimalarial drug","volume":"2014","author":"Shafiq N.","year":"2014","journal-title":"Malar. Res. Treat."},{"key":"e_1_2_5_12_1","first-page":"1024","article-title":"Hepatic metabolic studies of 1, 2, 4\u2010trioxanes derivative: A new antimalarial","volume":"2","author":"Gupta P.","year":"2014","journal-title":"J. Pharmacol. Clin. 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