{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:45:33Z","timestamp":1791297933165,"version":"4.1.0"},"reference-count":22,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2015,5,18]],"date-time":"2015-05-18T00:00:00Z","timestamp":1431907200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003246","name":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["project nr 727.011.004"],"award-info":[{"award-number":["project nr 727.011.004"]}],"id":[{"id":"10.13039\/501100003246","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001835","name":"Universiteit Maastricht","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001835","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:p>Matrix\u2010assisted laser desorption\/ionization\u2010mass spectrometry imaging (MALDI\u2010MSI) for the analysis of intact hair is a powerful tool for the detection of drugs of abuse in toxicology and forensic applications. Here we present a quick, easy, and reproducible method of preparing longitudinal sections of single hairs. This method improves the accessibility of chemicals embedded in the hair matrix for molecular imaging with mass spectrometry. The images obtained from a single, sectioned hair sample show molecular distributions in the exposed medulla, cortex, and a portion of the cuticle observed as a narrow layer surrounding the cortex. Using MALDI\u2010MS\/MS imaging, the distribution of cocaine was observed throughout five longitudinally sectioned drug\u2010user hair samples. The images showed the distribution of the product ion at <jats:italic>m<\/jats:italic>\/<jats:italic>z<\/jats:italic> 182, derived from the precursor ion of cocaine at <jats:italic>m<\/jats:italic>\/<jats:italic>z<\/jats:italic> 304. MetA\u2010SIMS images of longitudinally sectioned hair samples showed a more detailed distribution of cocaine at <jats:italic>m<\/jats:italic>\/<jats:italic>z<\/jats:italic> 304, benzoylecgonine the major metabolite of cocaine at <jats:italic>m<\/jats:italic>\/<jats:italic>z<\/jats:italic> 290 and other drugs such as methadone which was observed at <jats:italic>m<\/jats:italic>\/<jats:italic>z<\/jats:italic> 310. Chronological information of drug intake can be obtained more sensitively. The chronological detail is in hours rather than months, which is of great interest in clinical as well as forensic applications. Copyright \u00a9 2015 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/dta.1812","type":"journal-article","created":{"date-parts":[[2015,5,18]],"date-time":"2015-05-18T03:09:31Z","timestamp":1431918571000},"page":"859-865","source":"Crossref","is-referenced-by-count":62,"title":["Preparation of longitudinal sections of hair samples for the analysis of cocaine by MALDI\u2010MS\/MS and TOF\u2010SIMS imaging"],"prefix":"10.1002","volume":"7","author":[{"given":"Bryn","family":"Flinders","sequence":"first","affiliation":[{"name":"FOM\u2010Institute AMOLF  Science Park 104 1098 XG Amsterdam the Netherlands"},{"name":"M4I, The Maastricht MultiModal Molecular Imaging institute University of Maastricht  Universiteitssingel 50 6229 ER Maastricht the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Cuypers","sequence":"additional","affiliation":[{"name":"KU Leuven Toxicology and Pharmacology  Herestraat 49 PO 922, 3000 Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans","family":"Zeijlemaker","sequence":"additional","affiliation":[{"name":"FOM\u2010Institute AMOLF  Science Park 104 1098 XG Amsterdam the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Tytgat","sequence":"additional","affiliation":[{"name":"KU Leuven Toxicology and Pharmacology  Herestraat 49 PO 922, 3000 Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ron M. 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