{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T04:45:17Z","timestamp":1790743517894,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,7,1]],"date-time":"2016-07-01T00:00:00Z","timestamp":1467331200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:p>A rapid, simple and sensitive liquid chromatography\u2010tandem mass spectrometry (LC\u2010MS\/MS) method was developed for the qualitative and quantitative analysis of nine barbiturates (barbital, phenobarbital, pentobarbital, amobarbital, secobarbital, thiopental, butalbital, butabarbital, and hexobarbital) in human whole blood. Barbiturates were extracted from 100\u2009\u03bcL of human whole blood samples using a simple liquid\u2010liquid extraction (LLE) procedure, and detected by LC\u2010MS\/MS. An UPLC C18 (2.1\u2009mm\u2009\u00d7\u2009100\u2009mm, 1.7\u2009\u00b5m) column was used at 40\u2009\u00b0C for the separation and acetonitrile\/water system was used as the mobile phase with gradient elution. This method showed excellent accuracy (86\u2013111%) and precision (relative standard deviation &lt;15%). The limits of detection (LODs) were 0.2\u2009ng\/mL for barbital and secobarbital and 0.5\u2009ng\/mL for the other barbiturates. The linearity ranged from 2\u2009ng\/mL to 2000\u2009ng\/mL, with r<jats:sup>2<\/jats:sup>\u2009&gt;\u20090.99 over the range. This method achieved the separation and detection of pentobarbital and amobarbital at the same time in a convenient way. Moreover, it was both simple and sensitive for the determination of nine most commonly used barbiturate drugs, which was meaningful in the field of clinical and forensic toxicology. 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