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Tulathromycin was extracted from bison and deer sera with acetonitrile or trifluoroacetic acid and K<jats:sub>2<\/jats:sub>HPO<jats:sub>4<\/jats:sub> (pH 6.8) buffer solution and cleaned up on a conditioned Bond\u2010Elut cartridge. Tulathromycin, retained on the cartridge; it was eluted with methanol containing 2% formic acid, dried, re\u2010constituted in methanol\/1% formic acid, and analyzed by LC\u2010MS. The limit of quantification (LOQ) of the method was 0.6 ng\/mL in serum and 0.6 ng\/g in tissue with RSDs \u2264 10% and accurate over the linear calibration range of 0.8\u2013100 ng\/mL for bison serum, 0.6\u201350 ng\/mL for deer serum, 100\u20132500 ng\/g for deer muscle tissue, and 500\u20135000 ng\/g for deer lung tissue, all with coefficients of determination, r<jats:sup>2<\/jats:sup>\u22650.99. The validated method was used to quantify the concentration of tulathromycin residues in serum of bison and deer and selected tissue (lung and muscle tissue) samples obtained from 10 healthy, white\u2010tailed deer that were administered the therapeutic dose approved for cattle (i.e., a single 2.5 mg\/kg subcutaneous injection of tulathromycin in the neck). The deer were included in a tulathromycin drug depletion study. \u00a9 2016 Her Majesty the Queen in Right of Canada. Drug Testing and Analysis \u00a9 2016 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/dta.2018","type":"journal-article","created":{"date-parts":[[2016,7,22]],"date-time":"2016-07-22T06:10:31Z","timestamp":1469167831000},"page":"584-595","source":"Crossref","is-referenced-by-count":8,"title":["A single laboratory\u2010validated LC\u2010MS method for the analysis of tulathromycin residues in bison and deer sera and selected tissues of white\u2010tailed deer"],"prefix":"10.1002","volume":"8","author":[{"given":"Joe O.","family":"Boison","sequence":"first","affiliation":[{"name":"CFIA Saskatoon Laboratory  Saskatoon Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kali","family":"Bachtold","sequence":"additional","affiliation":[{"name":"College of Pharmacy, School of Graduate Studies University of Saskatchewan  Saskatoon Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johanna","family":"Matus","sequence":"additional","affiliation":[{"name":"Western College of Veterinary Medicine University of Saskatchewan  Saskatoon Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jane","family":"Alcorn","sequence":"additional","affiliation":[{"name":"College of Pharmacy, School of Graduate Studies University of Saskatchewan  Saskatoon Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Murray","family":"Woodbury","sequence":"additional","affiliation":[{"name":"Western College of Veterinary Medicine University of Saskatchewan  Saskatoon Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,7,22]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-40.2.366"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/104063870802000321"},{"key":"e_1_2_11_4_1","unstructured":"M.Woodbury.Mycoplasma infection in bison.2012 Available at:http:\/\/bisoncentre.com\/index.php\/producers\u20102\/resource\u2010library\/advanced\u2010bison\u2010information\/diseases\u2010of\u2010bison\u2010\/600\u2010mycoplasma[21 July 2015]."},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/104063871002200528"},{"key":"e_1_2_11_6_1","unstructured":"Drugs.com.Draxxin injectable solution (Canada). 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