{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T21:08:35Z","timestamp":1761512915969,"version":"build-2065373602"},"reference-count":15,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2010,12,29]],"date-time":"2010-12-29T00:00:00Z","timestamp":1293580800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2011,1]]},"abstract":"Abstract<\/jats:title>Methods have been developed to screen for and confirm darbepoetin alfa, recombinant human EPO, and methoxy polyethylene glycol\u2010epoetin ${\\bf{\\beta}}$<\/jats:styled-content> (PEG\u2010epoetin ${\\bf{\\beta}}$<\/jats:styled-content>) in horse plasma. All three methods screen samples with an enzyme\u2010linked immunosorbent assay (ELISA) and confirm by liquid chromatography\u2010tandem mass spectrometry (LC\u2010MS\/MS). This report focuses on PEG\u2010epoetin ${\\bf{\\beta}}$<\/jats:styled-content>. The ELISA assay was able to detect PEG\u2010epoetin ${\\bf{\\beta}}$<\/jats:styled-content> at 0.02 ng\/mL in 50 \u00b5L of horse plasma. Many samples had high background levels of immunoreactivity; however, introducing polyethylene glycol 6000 (PEG 6000) into the samples before the ELISA assay removed the high background and increased the apparent concentrations of PEG\u2010epoetin ${\\bf{\\beta}}$<\/jats:styled-content>. In samples collected following the administration of 100 \u00b5g of PEG\u2010epoetin ${\\bf{\\beta}}$<\/jats:styled-content> by the intravenous (IV), intramuscular (IM) and subcutaneous (SC) routes, PEG\u2010epoetin ${\\bf{\\beta}}$<\/jats:styled-content> was detectable up to 72, 144, and 120 h, respectively. The samples were prepared for LC\u2010MS\/MS analysis by extraction with anti\u2010rHuEPO\u2010antibodies\u2010coated Dynabeads followed by digestion with trypsin. The LC\u2010MS\/MS confirmation method used the multiple reaction monitoring (MRM) scan mode to monitor four precursor\u2010product ion transitions of the EPO\u2010derived peptide T6<\/jats:sub>. All four transitions of T6<\/jats:sub> were detectable with S\/N > 3. The limit of confirmation for PEG\u2010epoetin ${\\bf{\\beta}}$<\/jats:styled-content> was 1.0 ng\/mL in 2 mL of horse plasma. The method successfully confirmed the presence of PEG\u2010epoetin ${\\bf{\\beta}}$<\/jats:styled-content> in a sample collected from a Mircera\u00ae\u2010treated horse. Compared to PEG\u2010epoetin ${\\bf{\\beta}}$<\/jats:styled-content>, better sensitivity was achieved for darbepoetin alfa and recombinant human EPO. Darbepoetin alfa was detected in horse plasma four days after IM administration of 100 \u00b5g. Copyright \u00a9 2010 John Wiley & Sons, Ltd.<\/jats:p>","DOI":"10.1002\/dta.212","type":"journal-article","created":{"date-parts":[[2010,12,29]],"date-time":"2010-12-29T06:59:22Z","timestamp":1293605962000},"page":"68-73","source":"Crossref","is-referenced-by-count":18,"title":["Screen and confirmation of PEG\u2010epoetin \u03b2 in equine plasma"],"prefix":"10.1002","volume":"3","author":[{"given":"Y.","family":"Chang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. M.","family":"Maylin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Matsumoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. M.","family":"Neades","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. 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