{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,9]],"date-time":"2025-12-09T15:40:38Z","timestamp":1765294838958,"version":"build-2065373602"},"reference-count":12,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2017,2,24]],"date-time":"2017-02-24T00:00:00Z","timestamp":1487894400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:p>For the medico\u2010psychological assessment (MPA) during driving licence re\u2010granting in Germany, abstinence control including urine samples is required. In these programmes, even small amounts of markers for drug or alcohol abuse have to be detected. Thus, the concentrations of the target compounds are very low, and, in consequence, the sensitivity of the applied screening method has to be much higher than for clinical use. Modified drugs of abuse and ethyl glucuronide immunoassays on a Roche cobas c 501 analyzer were evaluated for precision, accuracy, onboard calibration stability, cross reactivity, sensitivity, and specificity using authentic urine samples. Precision (intra\u2010day and inter\u2010day relative standard deviation (RSD) and accuracy (bias) at three concentrations were 12% or lower for all parameters. The calibrations remained stable (deviations &lt;25%) for at least 28\u00a0days for all assays except amphetamines (21\u00a0days). Satisfactory cross reactivity was determined for the relevant analytes and also for several new psychoactive substances (NPS). The sensitivity was 100% for all parameters except methadone metabolite EDDP (92%) and fully met the sensitivity criteria for MPA urine testing. The presented kinetic interaction of microparticles in a solution (KIMS) immunoassays on a cobas c 501 thus provide a new method to reliably detect drug or alcohol consumption in abstinence control programmes requiring high sensitivity. Copyright \u00a9 2016 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/dta.2154","type":"journal-article","created":{"date-parts":[[2016,12,26]],"date-time":"2016-12-26T13:06:50Z","timestamp":1482757610000},"page":"1217-1223","source":"Crossref","is-referenced-by-count":5,"title":["Evaluation of KIMS immunoassays on a cobas c 501 analyzer for drugs of abuse and ethyl glucuronide testing in urine for forensic abstinence control"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3826-2327","authenticated-orcid":false,"given":"Merja A.","family":"Neukamm","sequence":"first","affiliation":[{"name":"Institute for Forensic Medicine, Medical Center \u2013 University of Freiburg, Faculty of Medicine, University of Freiburg  Albertstrasse 9, 79104 Freiburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arsham","family":"Bahrami","sequence":"additional","affiliation":[{"name":"Institute for Forensic Medicine, Medical Center \u2013 University of Freiburg, Faculty of Medicine, University of Freiburg  Albertstrasse 9, 79104 Freiburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1883-2804","authenticated-orcid":false,"given":"Volker","family":"Auw\u00e4rter","sequence":"additional","affiliation":[{"name":"Institute for Forensic Medicine, Medical Center \u2013 University of Freiburg, Faculty of Medicine, University of Freiburg  Albertstrasse 9, 79104 Freiburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Felix M.P.","family":"Mehne","sequence":"additional","affiliation":[{"name":"Roche Diagnostics GmbH  Nonnenwald 2, 82377 Penzberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva","family":"H\u00f6ss","sequence":"additional","affiliation":[{"name":"Roche Diagnostics GmbH  Nonnenwald 2, 82377 Penzberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,2,24]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"106","article-title":"Drug screening and confirmation by GC\u2013MS: Comparison of EMIT II and Online KIMS against 10 drugs between US and England laboratories","volume":"157","author":"Lu N. 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