{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T18:05:44Z","timestamp":1773511544836,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,12,18]],"date-time":"2017-12-18T00:00:00Z","timestamp":1513555200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The use of filtration\/pass\u2010through extraction (FPTE) and ultra\u2010performance liquid chromatography quadrupole time\u2010of\u2010flight mass spectrometry (UPLC\u2013qTOF\u2013MS) to detect tramadol (TRAM), dextromethorphan (DXM), and metabolites from skeletal remains is described. Rats (n=5) received 50 mg\/kg tramadol and were euthanized by CO<jats:sub>2<\/jats:sub> asphyxiation approximately 30 minutes post\u2010dose. Rats (n=4) received 75 mg\/kg dextromethorphan and were euthanized by CO<jats:sub>2<\/jats:sub> asphyxiation approximately 45 minutes post\u2010dose. Remains decomposed to skeleton outdoors and vertebral bones were collected. Bones were cleaned, dried, and pulverized to a fine powder. Bones underwent dynamic methanolic extraction followed by FPTE before analysis using UPLC\u2013qTOF\u2013MS. Recovery was at least 90% of maximal value within the first 10 minutes of methanolic extraction for all samples assayed. Analytical response was measured over the concentration range of 1\u2013500 ng\/mL, with precision and bias &lt;20% in triplicate analyses of all calibrators, and a limit of detection of 1 ng\/mL for TRAM, DXM, and all metabolites. The vertebral bone analyzed using this method detected TRAM, DXM, and their respective metabolites in all samples analyzed.<\/jats:p>","DOI":"10.1002\/dta.2327","type":"journal-article","created":{"date-parts":[[2017,10,20]],"date-time":"2017-10-20T23:36:43Z","timestamp":1508542603000},"page":"961-967","source":"Crossref","is-referenced-by-count":11,"title":["Semi\u2010quantitative analysis of tramadol, dextromethorphan, and metabolites in decomposed skeletal tissues by ultra performance liquid chromatography quadrupole time of flight mass spectrometry"],"prefix":"10.1002","volume":"10","author":[{"given":"H. M.","family":"Cornthwaite","sequence":"first","affiliation":[{"name":"Department of Chemistry &amp; Biochemistry Laurentian University  Sudbury Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Labine","sequence":"additional","affiliation":[{"name":"Department of Forensic Science Laurentian University  Sudbury Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8056-249X","authenticated-orcid":false,"given":"J. H.","family":"Watterson","sequence":"additional","affiliation":[{"name":"Department of Chemistry &amp; Biochemistry Laurentian University  Sudbury Ontario Canada"},{"name":"Department of Forensic Science Laurentian University  Sudbury Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,12,18]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003088-200443130-00004"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/00498250110057378"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(01)00388-7"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2009.04.042"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.2894"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(01)00237-7"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2003.11.034"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)00318-2"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.3870"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1527-3458.2007.00006.x"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2016.04.010"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(00)00609-5"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2003.12.009"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2007.09.017"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2004.09.040"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2004.11.049"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1570-0232(02)00797-3"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2004.12.031"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAF.0b013e31818738c9"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1520\/JFS2004067"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1520\/JFS15069J"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/34.8.510"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/bks045"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/bkt078"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/bku053"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/bkw083"},{"key":"e_1_2_7_28_1","first-page":"7","volume-title":"Standard Practices for Method Development in Forensic Toxicology","author":"SWGTOX","year":"2013"},{"key":"e_1_2_7_29_1","unstructured":"WiebeTR WattersonJH.Analysis of tramadol and O\u2010desmethyltramadol in decomposed skeletal tissues following acute and repeated tramadol exposure by gas chromatography mass spectrometry"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.1754"}],"container-title":["Drug Testing and Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdta.2327","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dta.2327","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T19:22:22Z","timestamp":1760383342000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/dta.2327"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,12,18]]},"references-count":29,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2018,6]]}},"alternative-id":["10.1002\/dta.2327"],"URL":"https:\/\/doi.org\/10.1002\/dta.2327","archive":["Portico"],"relation":{},"ISSN":["1942-7603","1942-7611"],"issn-type":[{"value":"1942-7603","type":"print"},{"value":"1942-7611","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,12,18]]}}}