{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T04:43:28Z","timestamp":1791434608799,"version":"4.3.3"},"reference-count":22,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2018,5,25]],"date-time":"2018-05-25T00:00:00Z","timestamp":1527206400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aims of this study were (1) to identify and quantify cocaine (COC), benzoylecgonine (BE), ecgonine methyl ester (EME), and cocaethylene (CE) in DBS; (2) to compare dried blood spots (DBSs) analytical results with the routine blood analyses; (3) to monitor analytes stability on DBS within a 3\u2010month period. Eighty\u2010five \u03bcL of blood from postmortem cases were put on a card for DBS analysis and kept in the dark, at room temperature. Samples were extracted through solid\u2010phase extraction (SPE) cartridges and injected in the liquid chromatography\u2013tandem mass spectrometry (LC\u2013MS\/MS) system. The analytical procedure is simple, sensitive, and specific. Limits of detection (LODs) and quantification (LOQs) were calculated at 1.0 and 5.0\u00a0ng\/mL(g) for COC and CE, and at 0.5 and 2\u00a0ng\/mL for EME and BE, respectively. Validation parameters fulfilled all the acceptance criteria. Fifty\u2010five postmortem cases were evaluated. Eighteen cases were positive for COC (44\u20132456\u00a0ng\/mL) and BE (228\u20134700\u00a0ng\/mL), 12 for EME (92\u20131500\u00a0ng\/mL), and 11 cases for CE (11\u2013273\u00a0ng\/mL). Stability was evaluated on 8 cases collected in the period January 2017\u2013January 2018. For each case, 5 DBSs were collected at T0. Four DBSs were analyzed within the 4 following weeks and 1 sample was analyzed after 3\u00a0months. The concentrations on DBSs, stored at room temperature, always matched the ones obtained on blood samples kept at \u221220\u00b0C (&lt;20% variation, both at T0 and after 3\u00a0months). BE and COC concentrations remained stable after a 3\u2010month storage, EME concentrations slightly increased after 3\u00a0weeks in the 2 analyzed samples, while CE provided a less homogeneous stability depending on the sample.<\/jats:p>","DOI":"10.1002\/dta.2399","type":"journal-article","created":{"date-parts":[[2018,5,4]],"date-time":"2018-05-04T04:01:49Z","timestamp":1525406509000},"page":"1430-1437","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["A liquid chromatography\u2013tandem mass spectrometry method for the determination of cocaine and metabolites in blood and in dried blood spots collected from postmortem samples and evaluation of the stability over a 3\u2010month period"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7420-9366","authenticated-orcid":false,"given":"Matteo","family":"Moretti","sequence":"first","affiliation":[{"name":"Department of Public Health, 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