{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T15:08:05Z","timestamp":1780412885250,"version":"3.54.1"},"reference-count":38,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2018,6,29]],"date-time":"2018-06-29T00:00:00Z","timestamp":1530230400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Spanish Ministry of Economy and Competitiveness (MINECO) and FEDER","award":["Project ref. CTQ2015-69899-R"],"award-info":[{"award-number":["Project ref. CTQ2015-69899-R"]}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Homeopathic products are still a controversial issue in modern medicine, understood as complementary or alternative medicine (CAM). In this particular case, homeopathic products prepared from <jats:styled-content style=\"fixed-case\"><jats:italic>Atropa belladonna<\/jats:italic><\/jats:styled-content> extracts may present specific problems due to the effects derived from its components. This article applies a simple, rapid, reliable method to the analysis of different homeopathic products obtained from <jats:styled-content style=\"fixed-case\"><jats:italic>Atropa belladonna<\/jats:italic><\/jats:styled-content>; drugs containing high concentration of plant extracts; and <jats:styled-content style=\"fixed-case\"><jats:italic>Atropa belladonna<\/jats:italic><\/jats:styled-content> seeds. The method was based on a simple solid\u2010phase preconcentration method followed by ultra\u2010high pressure liquid chromatography (UHPLC) coupled to high resolution mass spectrometry using Exactive\u2010Orbitrap as an analyser. An in\u2010house database was set and atropine and scopolamine were the compounds detected at highest concentrations in homeopathic products from <jats:styled-content style=\"fixed-case\"><jats:italic>Atropa belladonna<\/jats:italic><\/jats:styled-content> extracts (4.57 and 2.56\u00a0\u03bcg\/kg, respectively), in Belladonna ointment (4007 and 1139\u00a0\u03bcg\/kg, respectively) and Belladonna seeds (338 and 32.1\u00a0mg\/kg, respectively). Other tropane alkaloids such as tropine, apoatropine, aposcopolamine, tropinone, homatropine, and anisodamine were detected at lower concentrations (0.04\u20131.36\u00a0\u03bcg\/kg). When untargeted analysis was performed, other tropane alkaloids were identified in the tested samples, such as ecgonine (0.003\u00a0\u03bcg\/kg), benzoylecgonine (0.56\u00a0\u03bcg\/kg), calystegines A (19.6\u00a0\u03bcg\/kg), B (33.1\u00a0\u03bcg\/kg), and C (1.01\u00a0\u03bcg\/kg). Finally other compounds present in the homeopathic products, such as sugars (fructose, glucose, and lactose) or amino acids (valine, ornithine, leucine, and phenylalanine), were identified.<\/jats:p>","DOI":"10.1002\/dta.2416","type":"journal-article","created":{"date-parts":[[2018,5,28]],"date-time":"2018-05-28T23:34:32Z","timestamp":1527550472000},"page":"1579-1589","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Screening of drugs and homeopathic products from <scp><i>Atropa belladonna<\/i><\/scp> seed extracts: Tropane alkaloids determination and untargeted analysis"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4153-9788","authenticated-orcid":false,"given":"Jes\u00fas","family":"Mar\u00edn\u2010S\u00e1ez","sequence":"first","affiliation":[{"name":"Department of Chemistry and Physics, Analytical Chemistry Area University of Almer\u00eda, Research Centre for Agricultural and Food Biotechnology (BITAL), Agrifood Campus of International Excellence ceiA3  Almer\u00eda Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2505-2056","authenticated-orcid":false,"given":"Roberto","family":"Romero\u2010Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Physics, Analytical Chemistry Area University of Almer\u00eda, Research Centre for Agricultural and Food Biotechnology (BITAL), Agrifood Campus of International Excellence ceiA3  Almer\u00eda Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7904-7842","authenticated-orcid":false,"given":"Antonia","family":"Garrido Frenich","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Physics, Analytical Chemistry Area University of Almer\u00eda, Research Centre for Agricultural and Food Biotechnology (BITAL), Agrifood Campus of International Excellence ceiA3  Almer\u00eda Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco Javier","family":"Egea\u2010Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Physics, Analytical Chemistry Area University of Almer\u00eda, Research Centre for Agricultural and Food Biotechnology (BITAL), Agrifood Campus of International Excellence ceiA3  Almer\u00eda Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,6,29]]},"reference":[{"issue":"10","key":"e_1_2_6_2_1","first-page":"1","article-title":"Scientific Opinion on Tropane alkaloids in food and feed","volume":"11","author":"EFSA","year":"2013","journal-title":"EFSA J."},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/J157v07n01_08"},{"issue":"4","key":"e_1_2_6_4_1","first-page":"439","article-title":"Tropane alkaloids as medicinally useful natural products and their synthetic derivatives as new drugs","volume":"60","author":"Grynkiewicz G","year":"2008","journal-title":"Pharmacol Reports."},{"issue":"6","key":"e_1_2_6_5_1","first-page":"481","article-title":"Tropane alkaloids in pharmaceutical and phytochemical analysis","volume":"58","author":"Gadzikowska M","year":"2001","journal-title":"Acta Pol Pharm \u2013 Drug Res."},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-8741(84)90092-8"},{"key":"e_1_2_6_7_1","first-page":"717","volume-title":"Tropane alkaloids: Old Drugs Used in Modern Medicine","author":"Christen P","year":"2000"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2010.11.041"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1556\/AChrom.25.2013.3.10"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0731-7085(98)00223-4"},{"issue":"1","key":"e_1_2_6_11_1","first-page":"2","article-title":"The safe usage of herbal medicines: counter indications, cross\u2010reactivity and toxicity","volume":"5","author":"Cock I","year":"2015","journal-title":"Pharmacogn Commun."},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.homp.2004.07.004"},{"issue":"3","key":"e_1_2_6_13_1","first-page":"111","article-title":"Evaluation of anti\u2010inflammatory effect of Belladonna (a homeopathic drug) in experimental animals\u2010rats","volume":"4","author":"Vangoori Y","year":"2013","journal-title":"Int J Pharma Bio Sci."},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/joim.12676"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)67177-2"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/MJT.0b013e3182785eb7"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anpedi.2013.10.012"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2016.19090"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0379-0738(02)00162-7"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1365\/s10337-010-1524-y"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(01)82569-3"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/chir.22536"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpha.2011.09.008"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2013.12.006"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200700468"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2005.06.027"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1080\/19440049.2011.603704"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.1180"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.2132"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2017.08.052"},{"key":"e_1_2_6_31_1","unstructured":"ICH.ICH Topic Q2 (R1) Validation of Analytical Procedures: Text and Methodology.Int Conf Harmon.2005;1:1\u201313"},{"key":"e_1_2_6_32_1","unstructured":"European Commission.SANTE guidance document on analytical quality control and method validation procedures for pesticide residues and analysis in food and feed.2017;1:1\u201342."},{"issue":"12","key":"e_1_2_6_33_1","first-page":"1","article-title":"Occurrence of tropane alkaloids in food","volume":"13","author":"Mulder PPJ","year":"2016","journal-title":"EFSA Support Publ."},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(02)01387-0"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2015.03.005"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2014.07.066"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2016.09.075"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1200473109"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00438-015-1005-y"}],"container-title":["Drug Testing and Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdta.2416","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dta.2416","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T09:46:30Z","timestamp":1760348790000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/dta.2416"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,6,29]]},"references-count":38,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2018,10]]}},"alternative-id":["10.1002\/dta.2416"],"URL":"https:\/\/doi.org\/10.1002\/dta.2416","archive":["Portico"],"relation":{},"ISSN":["1942-7603","1942-7611"],"issn-type":[{"value":"1942-7603","type":"print"},{"value":"1942-7611","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,6,29]]},"assertion":[{"value":"2018-03-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-05-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-06-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}