{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T11:13:09Z","timestamp":1781521989734,"version":"3.54.1"},"reference-count":33,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2018,7,5]],"date-time":"2018-07-05T00:00:00Z","timestamp":1530748800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003965","name":"International Olympic Committee","doi-asserted-by":"publisher","award":["2014 IOC Anti\u2010Doping Research Fund"],"award-info":[{"award-number":["2014 IOC Anti\u2010Doping Research Fund"]}],"id":[{"id":"10.13039\/501100003965","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","award":["DEP2012\u201032048"],"award-info":[{"award-number":["DEP2012\u201032048"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The use of dried blood spots (DBS) for anti\u2010doping purposes would facilitate an increase in the number of blood samples because it eliminates the need for specialized personnel and involves minimal invasiveness, reduced costs, stability, and easy transportation and storage. Here, the electrophoretic methodology established by the World Anti\u2010Doping Agency (WADA) to detect erythropoiesis\u2010stimulating agents (ESAs) has been adapted to evaluate their applicability to DBS. A qualitative procedure to detect recombinant erythropoietin (rEPO), novel erythropoiesis\u2010stimulating protein (NESP), and continuous erythropoietin receptor activator (CERA) in a single DBS was optimized and validated. For rEPO and NESP, confirmation was performed in finger\u2010prick DBS from a pilot study and an administration patients study, respectively. For CERA, detection capabilities were evaluated in DBS prepared with modeled\u2010blood spiked with known concentrations of the protein. Main validation parameters concerning DBS sampling such as stability, hematocrit influence, and blood type (capillary vs. venous) described minor variations. Onsite drying appeared not to be essential before transport. Intra\u2010 and inter\u2010day variation range was 2.9%\u201323.5%. Linearity was maintained (<jats:italic>r<\/jats:italic>\u00a0\u2265\u00a00.9) and ESAs were robustly recovered (CV\u00a0\u2264\u00a020.2%). The validated method permitted the detection of treated subjects after 48\u00a0hours and 17\u00a0days of rEPO and NESP administration, respectively. The reproduction of a CERA pharmacokinetics showed good possibilities for the method with a detection window that could reach 16\u00a0days after its actual administration. Thus, results provided here reinforce the suitability of DBS blood sampling for the analysis of ESA misuse in sports drug testing.<\/jats:p>","DOI":"10.1002\/dta.2418","type":"journal-article","created":{"date-parts":[[2018,6,6]],"date-time":"2018-06-06T23:18:21Z","timestamp":1528327101000},"page":"1496-1507","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Detection of erythropoiesis\u2010stimulating agents in a single dried blood spot"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1902-9973","authenticated-orcid":false,"given":"Gemma","family":"Reverter\u2010Branchat","sequence":"first","affiliation":[{"name":"Integrative Pharmacology and Systems Neuroscience Research Group, Neurosciences Research Program IMIM \u2013 Hospital del Mar Medical Research Institute  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa","family":"Ventura","sequence":"additional","affiliation":[{"name":"Catalonian Antidoping Laboratory, Doping Control Research Group, Neurosciences Research Programme IMIM \u2013 Hospital del Mar Medical Research Institute  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed","family":"Ezzel Din","sequence":"additional","affiliation":[{"name":"Integrative Pharmacology and Systems Neuroscience Research Group, Neurosciences Research Program IMIM \u2013 Hospital del Mar Medical Research Institute  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juli\u00e1n","family":"Mateus","sequence":"additional","affiliation":[{"name":"Integrative Pharmacology and Systems Neuroscience Research Group, Neurosciences Research Program IMIM \u2013 Hospital del Mar Medical Research Institute  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carme","family":"Pedro","sequence":"additional","affiliation":[{"name":"Department of Hematology Hospital del Mar\u2010IMIM  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordi","family":"Segura","sequence":"additional","affiliation":[{"name":"Integrative Pharmacology and Systems Neuroscience Research Group, Neurosciences Research Program IMIM \u2013 Hospital del Mar Medical Research Institute  Barcelona Spain"},{"name":"Catalonian Antidoping Laboratory, Doping Control Research Group, Neurosciences Research Programme IMIM \u2013 Hospital del Mar Medical Research Institute  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,7,5]]},"reference":[{"key":"e_1_2_6_2_1","unstructured":"WADA.World Anti\u2010Doping Agency (WADA).https:\/\/www.wada\u2010ama.org\/. 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