{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T02:48:54Z","timestamp":1790304534934,"version":"4.1.0"},"reference-count":15,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,7,17]],"date-time":"2018-07-17T00:00:00Z","timestamp":1531785600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>An athlete contested an adverse analytical finding involving hydrochlorothiazide, and requested our laboratory for testing his hair. As there is no reference in the literature about identification of hydrochlorothiazide in hair, several volunteers were first enrolled (4 after a single 25\u00a0mg administration and 10 with daily therapeutic treatment). A specific method was developed by ultra\u2010performance liquid chromatography\u2013tandem mass spectrometry (UPLC\u2013MS\/MS. Hair samples were decontaminated with dichloromethane and 30\u00a0mg were incubated in buffer at pH\u00a07.0 for 15\u00a0hours at 50\u00b0C. Then, 5\u00a0mL ethyl acetate was added for extraction. Linearity was observed for hydrochlorothiazide concentrations ranging from 5 to 2000\u00a0pg\/mg. The limit of quantification was 5\u00a0pg\/mg. The coefficients of variation (CVs) of repeatability and matrix effect were lower than 20%. Analysis of the 0\u20132\u2010cm segment of the 4 volunteers having received a single dose, collected 1\u00a0month after administration, was negative at the limit of quantification. The hair of the 10 patients (proximal 2\u00a0cm) on daily treatment was positive with concentrations ranging from 12 to 1845\u00a0pg\/mg, with no correlation between daily dose and concentration. The athlete's hair tested positive for hydrochlorothiazide at 36\u00a0pg\/mg in the segment corresponding to the period of the urinary control. Since a single exposure to hydrochlorothiazide is not detectable in hair and based on the results of the patients on daily treatment, the concentration found in the athlete has been interpreted as corresponding to repeated exposures, where it was not possible to establish the dosage and the frequency.<\/jats:p>","DOI":"10.1002\/dta.2445","type":"journal-article","created":{"date-parts":[[2018,6,19]],"date-time":"2018-06-19T16:30:32Z","timestamp":1529425832000},"page":"157-161","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["First identification of a diuretic, hydrochlorothiazide, in hair: Application to a doping case and interpretation of the results"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7804-3946","authenticated-orcid":false,"given":"L.","family":"Gheddar","sequence":"first","affiliation":[{"name":"Institut de m\u00e9decine l\u00e9gale, laboratoire de toxicologie  Strasbourg France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JS.","family":"Raul","sequence":"additional","affiliation":[{"name":"Institut de m\u00e9decine l\u00e9gale, laboratoire de toxicologie  Strasbourg France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4174-5362","authenticated-orcid":false,"given":"P.","family":"Kintz","sequence":"additional","affiliation":[{"name":"Institut de m\u00e9decine l\u00e9gale, laboratoire de toxicologie  Strasbourg France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,7,17]]},"reference":[{"key":"e_1_2_5_2_1","first-page":"1049","volume-title":"Disposition of Toxic Drug and Chemicals in Man","author":"Baselt RC","year":"2017"},{"key":"e_1_2_5_3_1","unstructured":"World Anti\u2010Doping Agency.What is prohibited.https:\/\/www.wada\u2010ama.org\/en\/content\/what\u2010is\u2010prohibited\/prohibited\u2010at\u2010all\u2010times\/diuretics\u2010and\u2010masking\u2010agents. Accessed February 27 2018."},{"key":"e_1_2_5_4_1","unstructured":"World Anti\u2010Doping Agency.World Anti\u2010Doping Code International Standard Laboratories.June 2016. Montreal Version9.https:\/\/www.wada\u2010ama.org\/sites\/default\/files\/resources\/files\/isl_june_2016.pdf. Accessed May 05 2018."},{"key":"e_1_2_5_5_1","first-page":"3","article-title":"Consensus of the society of hair testing on hair testing for doping agents","volume":"107","author":"Sachs H","year":"2000","journal-title":"Forensic Sci Int"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.forsciint.2011.10.018"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0379-0738(99)00178-4"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0379-0738(99)00176-0"},{"issue":"36","key":"e_1_2_5_9_1","first-page":"5480","article-title":"Hair analysis in forensic toxicology: an updated review with special focus on pitfalls","volume":"23","author":"Kintz P","year":"2017","journal-title":"Curr Pharm Des"},{"key":"e_1_2_5_10_1","first-page":"95","volume-title":"Analytical and Practical Aspects of Drug Testing in Hair. Forensic science series","author":"Jurado C","year":"2007"},{"key":"e_1_2_5_11_1","first-page":"1","volume-title":"Analytical and Practical Aspects of Drug Testing in Hair. Forensic science series","author":"Kronstrand R","year":"2007"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/20.1.1"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6904-1-2"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1520\/JFS2002209"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/js960268h"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.1746"}],"container-title":["Drug Testing and Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdta.2445","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dta.2445","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T14:00:41Z","timestamp":1760364041000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/dta.2445"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,7,17]]},"references-count":15,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,1]]}},"alternative-id":["10.1002\/dta.2445"],"URL":"https:\/\/doi.org\/10.1002\/dta.2445","archive":["Portico"],"relation":{},"ISSN":["1942-7603","1942-7611"],"issn-type":[{"value":"1942-7603","type":"print"},{"value":"1942-7611","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,7,17]]},"assertion":[{"value":"2018-03-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-06-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-07-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}