{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T11:50:39Z","timestamp":1782906639834,"version":"3.54.5"},"reference-count":23,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,11,26]],"date-time":"2018-11-26T00:00:00Z","timestamp":1543190400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP16K09211"],"award-info":[{"award-number":["JP16K09211"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP24590859"],"award-info":[{"award-number":["JP24590859"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The misuse of psychotropic drugs intended for medical treatment represents a recent worldwide public health concern. Quenchbody (Q\u2010body) is a novel fluoroimmunosensor that can detect an antigen immediately without additional reagents or washing steps. Here, we describe creating Q\u2010bodies for the detection of the antidepressant fluvoxamine (FLV) and determining optimal conditions to achieve the highest fluorescence intensity (FI). We prepared five Q\u2010bodies with the fluorophore labeled at either the N\u2010 or C\u2010 terminus and with different linker lengths. Fluorescence was measurable within minutes, indicating the interaction of Q\u2010bodies with FLV. The normalized FI (FI ratio) of the N\u2010terminus labeled Q\u2010body increased approximately 1.5\u2010fold upon FLV addition; Q\u2010bodies labeled at the C\u2010terminus did not significantly increase FI. Among the fluorescence dyes used in this study, Rhodamine 6G labeled Q\u2010body showed the best FI ratio. EC<jats:sub>50<\/jats:sub> values of the N\u2010terminus labeled Q\u2010bodies were similar (23.2\u2013224nM) regardless of linker length or labeling dye. We examined whether the Q\u2010body could be applicable to serum matrix instead of phosphate\u2010buffered saline. The intact serum interfered strongly with the Q\u2010body fluorescence. However, the FI ratios of the Q\u2010body for FLV\u2010spiked serum filtrate, for which proteins were removed by filtration, showed a dose\u2010dependency for detecting FLV levels. Deproteinization, which does not interfere with Q\u2010body fluorescence measurements, is likely necessary to detect serum FLV with high sensitivity. This study demonstrates the potential of Q\u2010body probes as a tool towards developing creative immunoassay applications.<\/jats:p>","DOI":"10.1002\/dta.2520","type":"journal-article","created":{"date-parts":[[2018,10,17]],"date-time":"2018-10-17T05:11:09Z","timestamp":1539753069000},"page":"601-609","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Development of a fluvoxamine detection system using a Quenchbody, a novel fluorescent biosensor"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0221-0645","authenticated-orcid":false,"given":"Ako","family":"Sasao","sequence":"first","affiliation":[{"name":"Department of Forensic Medicine, Faculty of Life Sciences Kumamoto University  Kumamoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michiyo","family":"Takaki","sequence":"additional","affiliation":[{"name":"Department of Forensic Medicine, Faculty of Life Sciences Kumamoto University  Kumamoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hee\u2010Jin","family":"Jeong","sequence":"additional","affiliation":[{"name":"Department of Biological and Chemical Engineering Hongik University  Sejong\u2010si South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kosei","family":"Yonemitsu","sequence":"additional","affiliation":[{"name":"Department of Forensic Medicine, Faculty of Life Sciences Kumamoto University  Kumamoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuki","family":"Ohtsu","sequence":"additional","affiliation":[{"name":"Department of Forensic Medicine, Faculty of Life Sciences Kumamoto University  Kumamoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Tsutsumi","sequence":"additional","affiliation":[{"name":"Department of Forensic Medicine, Faculty of Life Sciences Kumamoto University  Kumamoto 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