{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T16:51:31Z","timestamp":1792083091461,"version":"4.4.0"},"reference-count":14,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,5,8]],"date-time":"2019-05-08T00:00:00Z","timestamp":1557273600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>CJC\u20101295 is a peptide\u2010based drug that stimulates the production of growth hormone (GH) from the pituitary gland. It incorporates a functional maleimido group at the C\u2010terminus that allows it to covalently bind plasma proteins such as serum albumin. These CJC\u20101295\u2010protein conjugates have a much greater half\u2010life compared to the unconjugated peptide and are capable of stimulating GH production for more than six days in humans after a single administration. Conjugated CJC\u20101295 is difficult to detect in blood by mass spectrometry due to its low abundance, high molecular weight, and conjugation to a range of different protein substrates. Previously we described a screening procedure for the detection of CJC\u20101295 in equine plasma using an immuno\u2010PCR assay. Here we demonstrate the confirmation of CJC\u20101295 in equine plasma by LC\u2212MS\/MS after immuno\u2010affinity capture and tryptic digestion. Using this method, CJC\u20101295 was identified down to concentrations as low as 180\u00a0pg\/mL in 1\u00a0mL of equine plasma.<\/jats:p>","DOI":"10.1002\/dta.2599","type":"journal-article","created":{"date-parts":[[2019,4,1]],"date-time":"2019-04-01T20:58:45Z","timestamp":1554152325000},"page":"1248-1257","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A method for confirming CJC\u20101295 abuse in equine plasma samples by LC\u2013MS\/MS"],"prefix":"10.1002","volume":"11","author":[{"given":"Mark","family":"Timms","sequence":"first","affiliation":[{"name":"Biological Research Unit Racing Analytical Services Ltd  Flemington Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine","family":"Ganio","sequence":"additional","affiliation":[{"name":"Biological Research Unit Racing Analytical Services Ltd  Flemington Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0423-6120","authenticated-orcid":false,"given":"Rohan","family":"Steel","sequence":"additional","affiliation":[{"name":"Biological Research Unit Racing Analytical Services Ltd  Flemington Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,5,8]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2004-1286"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(84)91647-4"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2005-1536"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-016-9377-3"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2011.08.009"},{"issue":"4","key":"e_1_2_5_7_1","article-title":"An Immuno\u2010PCR screen for the detection of CJC\u20101295 and other growth hormone releasing hormone analogues in equine plasma","volume":"11","author":"Timms M","year":"2019","journal-title":"Drug Test Anal"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac070135o"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-010-3455-8"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.1835"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.6b01060"},{"key":"e_1_2_5_12_1","unstructured":"AORC MS criteria (modified 23 Aug 16) \u2010 Association of Official Racing Chemists.http:\/\/www.aorc\u2010online.org\/documents\/aorc\u2010ms\u2010criteria\u2010modified\u201023\u2010aug\u201016\/. 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Accessed 18th October 2018"}],"container-title":["Drug Testing and Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dta.2599","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/dta.2599","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dta.2599","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T22:03:28Z","timestamp":1760393008000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/dta.2599"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,5,8]]},"references-count":14,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2019,8]]}},"alternative-id":["10.1002\/dta.2599"],"URL":"https:\/\/doi.org\/10.1002\/dta.2599","archive":["Portico"],"relation":{},"ISSN":["1942-7603","1942-7611"],"issn-type":[{"value":"1942-7603","type":"print"},{"value":"1942-7611","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,5,8]]},"assertion":[{"value":"2018-11-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-05-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}