{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T04:43:31Z","timestamp":1791434611858,"version":"4.3.3"},"reference-count":29,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,6,20]],"date-time":"2019-06-20T00:00:00Z","timestamp":1560988800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aims of this study were (a) to develop a liquid chromatography\u2212tandem mass spectrometry (LC\u2013MS\/MS) method for the identification of 27 benzodiazepines and metabolites in dried blood spots (DBSs) and in whole blood; (b) to compare the diagnostic reliability of DBSs with blood analyses; and c) to monitor analytes stability on DBSs within a three\u2010month period. Aliquots of 85\u00a0\u03bcL of blood from post\u2010mortem cases were pipetted on cards for DBS analysis. We also collected a tube of blood and stored it at\u00a0\u2212\u00a020\u00b0C. The cards were allowed to dry at room temperature. For each case, DBSs were analyzed immediately (T0), within the following 3\u00a0weeks (T1, T2, T3) and after 3\u00a0months (T4). The method was applied to 60 post\u2010mortem cases. A screening procedure was applied to all 27 molecules, while the method was fully validated for the 9 molecules detected in real samples. Limits of detection (LODs) and limits of quantification (LOQs) were in the range 0.1\u201350.0\u00a0ng\/mL and 5.0\u2013100.0\u00a0ng\/mL, respectively. Nine analytes were detected and quantitated in 14 cases: diazepam (<jats:italic>n<\/jats:italic>\u00a0=\u00a06, 58\u2013162\u00a0ng\/mL), desmethyldiazepam (<jats:italic>n<\/jats:italic>\u00a0=\u00a06, 99\u2010\u00a0&gt;\u00a0500\u00a0ng\/mL), 7\u2010aminoclonazepam (<jats:italic>n<\/jats:italic>\u00a0=\u00a05, 43\u2010\u00a0&gt;\u00a0500\u00a0ng\/mL), alprazolam (<jats:italic>n<\/jats:italic>\u00a0=\u00a02, 15 and 69\u00a0ng\/mL), chlordesmethyldiazepam (<jats:italic>n<\/jats:italic>\u00a0=\u00a01, 55\u00a0ng\/mL), desalkylflurazepam (<jats:italic>n<\/jats:italic>\u00a0=\u00a01, 270\u00a0ng\/mL), flurazepam (<jats:italic>n<\/jats:italic>\u00a0=\u00a01, 50\u00a0ng\/mL), medazepam (<jats:italic>n<\/jats:italic>\u00a0=\u00a01, 155\u00a0ng\/mL), and midazolam (<jats:italic>n<\/jats:italic>\u00a0=\u00a01, 227\u00a0ng\/mL). The concentrations on DBSs were in good agreement with those obtained on conventional blood samples (&lt;20% variation). Except for midazolam (degraded in 1\u00a0week), desalkylflurazepam and medazepam (decreased more than 50% after three months), a good stability for a three\u2010month period was observed for most of the compounds detected in real post\u2010mortem samples.<\/jats:p>","DOI":"10.1002\/dta.2653","type":"journal-article","created":{"date-parts":[[2019,5,24]],"date-time":"2019-05-24T13:05:08Z","timestamp":1558703108000},"page":"1403-1411","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Determination of benzodiazepines in blood and in dried blood spots collected from post\u2010mortem samples and evaluation of the stability over a three\u2010month period"],"prefix":"10.1002","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7420-9366","authenticated-orcid":false,"given":"Matteo","family":"Moretti","sequence":"first","affiliation":[{"name":"Department of Public Health, Experimental and Forensic Medicine 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Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia Damiana","family":"Vison\u00e0","sequence":"additional","affiliation":[{"name":"Department of Public Health, Experimental and Forensic Medicine University of Pavia  Pavia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"Tajana","sequence":"additional","affiliation":[{"name":"Department of Public Health, Experimental and Forensic Medicine University of Pavia  Pavia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio Marco Maria","family":"Osculati","sequence":"additional","affiliation":[{"name":"Department of Public Health, Experimental and Forensic Medicine University of Pavia  Pavia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4584-5552","authenticated-orcid":false,"given":"Luca","family":"Morini","sequence":"additional","affiliation":[{"name":"Department of Public Health, Experimental 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