{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T20:04:55Z","timestamp":1788465895440,"version":"build-2803163510"},"reference-count":13,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,1,10]],"date-time":"2020-01-10T00:00:00Z","timestamp":1578614400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Luteinizing hormone (LH) is a dimeric glycoprotein produced and secreted by the pituitary gland, with a molecular weight of approximately 30 000 Da. The main clinical use for exogenous LH\u2010administration is typically linked to the treatment of infertility, in both men and women. The desired effect of LH misuse in sport is due to the enhancement of testicular production of testosterone. Elevated LH levels may also indicate the usage of gonadotropin\u2010releasing factors or estrogen blockers. Therefore, LH is listed by the World Anti\u2010Doping Agency (WADA) as a prohibited substance in male athletes, and according to the WADA technical document, laboratories should determine the the total LH concentrations in urine. The TD lists two different assays that are suitable for measuring total LH in urine, Delfia and Siemens Immulite. Other fit\u2010for\u2010purpose assays can be used, as long as they are capable of detecting total LH in urine. In case an assay not listed in the TD will be used, population\u2010based reference values have to be determined in the validation procedure.<\/jats:p><jats:p>In this study a new immunoassay was validated for the measurement of LH in urine. The assay (AccuBind ELISA Microwells, Luteinizing Hormone, Monobind Inc.), originally intended for serum, showed adequate sensitivity and was proven fit\u2010for\u2010purpose in routine doping control. Population\u2010based distribution of the assay was in good agreement with the results of Delfia and Immulite assays, for which the method\u2010specific cut\u2010off\u2010values are 40 IU\/L (Delfia) and 60 IU\/L (Siemens Immulite).<\/jats:p>","DOI":"10.1002\/dta.2716","type":"journal-article","created":{"date-parts":[[2019,10,26]],"date-time":"2019-10-26T17:18:07Z","timestamp":1572110287000},"page":"239-246","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Analysis of luteinizing hormone (LH): Validation of a commercial ELISA kit for LH analysis and quantification in doping control samples"],"prefix":"10.1002","volume":"12","author":[{"given":"Riika","family":"M\u00e4kel\u00e4","sequence":"first","affiliation":[{"name":"Doping control laboratory United Medix laboratories"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antti","family":"Leinonen","sequence":"additional","affiliation":[{"name":"Doping control laboratory United Medix laboratories"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6516-2412","authenticated-orcid":false,"given":"Tina","family":"Suominen","sequence":"additional","affiliation":[{"name":"Doping control laboratory United Medix laboratories"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,1,10]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0303-7207(96)03942-1"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2013.12.009"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjp.2008.102"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2265.2008.03516.x"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2014.01.011"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.94"},{"key":"e_1_2_5_8_1","unstructured":"Reporting & Management of urinary human chorionic gonadotrophin (hCG) and luteinizing hormone (LH) findings in male athletes. WADA Technical Document \u2013 TD2019CG\/LH 2019"},{"issue":"11","key":"e_1_2_5_9_1","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1002\/dta.71","article-title":"Screening for testosterone abuse in male athletes using the measurement of urinary LH, a revision of the paradigm","volume":"1","author":"Goebel C","year":"2009","journal-title":"Drug Test Anal"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/43.11.2091"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1310147"},{"key":"e_1_2_5_12_1","unstructured":"StenmanU\u2010H AlfthanH KuuranneT LeinonenA. Reference values for luteinizing hormone in urine. Final report. WADA funded research project. Submitted to WADA in 2018."},{"key":"e_1_2_5_13_1","unstructured":"Decision limits for the confirmatory quantification of threshold substances. WADA Technical Document \u2013 TD2019DL v. 2.0"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/13.4.826"}],"container-title":["Drug Testing and Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdta.2716","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dta.2716","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/dta.2716","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dta.2716","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T22:59:32Z","timestamp":1760396372000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/dta.2716"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,10]]},"references-count":13,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2020,2]]}},"alternative-id":["10.1002\/dta.2716"],"URL":"https:\/\/doi.org\/10.1002\/dta.2716","archive":["Portico"],"relation":{},"ISSN":["1942-7603","1942-7611"],"issn-type":[{"value":"1942-7603","type":"print"},{"value":"1942-7611","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,10]]},"assertion":[{"value":"2018-12-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-10-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-01-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}