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However, each oxime has characteristic agent\u2010dependent reactivating efficacy, and therefore the combined administration of complementary oximes might be a promising approach to improve therapy. Accordingly, a new high\u2010performance liquid chromatography method with diode\u2010array detection (HPLC\u2010DAD) was developed and validated allowing for simultaneous or single quantification of 2\u2010PAM, Obi, and HI\u20106 in human plasma. Plasma was precipitated using 5% w\/v aqueous zinc sulfate solution and subsequently acetonitrile yielding high recoveries of 94.2%\u2013101.0%. An Atlantis T3 column (150 \u00d7 2.1mm I.D., 3 \u03bcm) was used for chromatographic separation with a total run time of 15 min. Quantification was possible without interferences within a linear range from 0.12 to 120 \u03bcg\/mL for all oximes. Excellent intra\u2010day (accuracy 91.7%\u201398.6%, precision 0.5%\u20134.4%) and inter\u2010day characteristics (accuracy 89.4%\u201397.4%, precision 0.4%\u20132.2%) as well as good ruggedness were found. Oximes in processed samples were stable for at least 12 h in the autosampler at 15\u00b0C as well as in human plasma for at least four freeze\u2013thaw cycles. Finally, the method was applied to plasma samples of a clinical case of pesticide poisoning.<\/jats:p>","DOI":"10.1002\/dta.2800","type":"journal-article","created":{"date-parts":[[2020,4,8]],"date-time":"2020-04-08T09:44:41Z","timestamp":1586339081000},"page":"938-947","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["A novel high\u2010performance liquid chromatography with diode array detector method for the simultaneous quantification of the enzyme\u2010reactivating oximes obidoxime, pralidoxime, and HI\u20106 in human plasma"],"prefix":"10.1002","volume":"12","author":[{"given":"Tamara","family":"Kranawetvogl","sequence":"first","affiliation":[{"name":"Bundeswehr Institute of Pharmacology and Toxicology  Munich Germany"},{"name":"Walther\u2010Straub\u2010Institut, Ludwig\u2010Maximilians\u2010Universit\u00e4t  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dirk","family":"Steinritz","sequence":"additional","affiliation":[{"name":"Bundeswehr Institute of Pharmacology and Toxicology  Munich Germany"},{"name":"Walther\u2010Straub\u2010Institut, Ludwig\u2010Maximilians\u2010Universit\u00e4t  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Horst","family":"Thiermann","sequence":"additional","affiliation":[{"name":"Bundeswehr Institute of Pharmacology and Toxicology  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7126-6709","authenticated-orcid":false,"given":"Harald","family":"John","sequence":"additional","affiliation":[{"name":"Bundeswehr Institute of Pharmacology and Toxicology  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,4,23]]},"reference":[{"key":"e_1_2_5_2_1","first-page":"527","volume-title":"Experimental and Clinical Neurotoxicology","author":"Davis C","year":"1980"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/0885066603258659"},{"issue":"1","key":"e_1_2_5_4_1","first-page":"289","article-title":"Cholinesterase inhibition by organophosphorus compounds and its clinical effects","volume":"44","author":"Namba T","year":"1971","journal-title":"Bull World Health Organ"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/qjmed\/93.11.715"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)67598-8"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2458-7-357"},{"key":"e_1_2_5_8_1","first-page":"615","article-title":"Survey of pesticide poisoning in Sri Lanka","volume":"60","author":"Jeyaratnam J","year":"1982","journal-title":"Bull World Health Organ"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0277-9536(97)00193-7"},{"key":"e_1_2_5_10_1","unstructured":"General Assembly Security Council.United Nations mission to investigate allegations of the use of chemical weapons in the Syrian Arab Republic. 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