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Twenty milligrams of hair was decontaminated in dichloromethane and underwent liquid extraction. 10 \u03bcL of the reconstituted residue were injected onto the system. The separation was performed in 12 minutes in a gradient mode at a flow rate of 300 \u03bcL\/min using a Hypersyl Gold PFP column (100 \u00d7 2.1 mm i.d., 1.9 \u03bcm) maintained at 30\u00b0C. Compounds were detected in positive ionization and MRM modes using a TSQ Endura mass spectrometer (ThermoFisher). The method was validated according to EMA guidelines. The LLOQ was in the range 1\u201350 pg\/mg, and the calibration ranged from the LLOQ\u20101000 pg\/mg. Intra\u2010 and inter\u2010day accuracy (bias) and precision were &lt; 15%. Extraction recoveries of parent drugs and metabolites were 74\u2013120% and 7\u201362%, respectively. The matrix effect was in the range 59\u2013126% (CVs \u2264 12.9%). Fentanyl was found in six cases at concentrations of &lt; 1\u20131650 pg\/mg (n = 14 segments). Five fentanyl analogs were quantified in two cases: 3\u2010fluorofentanyl (25\u2013150 pg\/mg, n = 5), furanylfentanyl (15\u2013500 pg\/mg, n = 5), methoxyacetylfentanyl (500\u2013600 pg\/mg, n = 2), acetylfentanyl (1 pg\/mg, n = 2), carfentanyl (2.5\u20133 pg\/mg, n = 2). This fully validated method allowed us to test for the first time 3\u2010fluorofentanyl and norcarfentanil in hair among 15 other NSOs, and brings new data regarding 3\u2010fluorofentanyl and methoxyacetylfentanyl hair concentrations.<\/jats:p>","DOI":"10.1002\/dta.2868","type":"journal-article","created":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T03:08:16Z","timestamp":1590980896000},"page":"1298-1308","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Development and validation of liquid chromatography\u2010tandem mass spectrometry targeted screening of 16 fentanyl analogs and U\u201047700 in hair: Application to 137 authentic samples"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9015-5342","authenticated-orcid":false,"given":"Islam Amine","family":"Larabi","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology Paris\u2010Saclay University (Versailles Saint\u2010Quentin\u2010en\u2010Yvelines University), Inserm U\u20101173, Raymond Poincar\u00e9 Hospital, AP\u2010HP  Garches France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Martin","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology Paris\u2010Saclay University (Versailles Saint\u2010Quentin\u2010en\u2010Yvelines University), Inserm U\u20101173, Raymond Poincar\u00e9 Hospital, AP\u2010HP  Garches France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Etting","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology Paris\u2010Saclay University (Versailles Saint\u2010Quentin\u2010en\u2010Yvelines University), Inserm U\u20101173, Raymond Poincar\u00e9 Hospital, AP\u2010HP  Garches France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory","family":"Pfau","sequence":"additional","affiliation":[{"name":"Addiction clinic Piti\u00e9 Salp\u00e9tri\u00e8re hospital, AP\u2010HP  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yves","family":"Edel","sequence":"additional","affiliation":[{"name":"Addiction clinic Piti\u00e9 Salp\u00e9tri\u00e8re hospital, AP\u2010HP  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean Claude","family":"Alvarez","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology Paris\u2010Saclay University (Versailles Saint\u2010Quentin\u2010en\u2010Yvelines University), Inserm U\u20101173, Raymond Poincar\u00e9 Hospital, AP\u2010HP  Garches France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,7,7]]},"reference":[{"key":"e_1_2_7_2_1","unstructured":"Products \u2010 Data Briefs \u2010 Number 329 \u2010 November 2018.https:\/\/www.cdc.gov\/nchs\/products\/databriefs\/db329.htm. 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