{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T11:38:23Z","timestamp":1788349103458,"version":"build-2803163510"},"reference-count":29,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2020,7,16]],"date-time":"2020-07-16T00:00:00Z","timestamp":1594857600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The amphetamine molecule contains a chiral center and its enantiomers exhibit differences in pharmacological effects, with the S\u2010enantiomer mediating most of the central nervous system stimulating activity. The majority of prescribed amphetamine consists of the pure S\u2010enantiomer, but therapeutic formulations containing the R\u2010enantiomer in various proportions are also available. Illegal amphetamine remains available mainly as a racemic mixture of the R\u2010 and S\u2010enantiomers. To distinguish between legal and illegal consumption of amphetamine a method for enantiomeric separation and quantification of R\/S\u2010amphetamine in serum was developed and validated using ultra\u2010high performance supercritical fluid chromatography\u2010tandem mass spectrometry (UHPSFC\u2010MS\/MS). Sample preparation prior to UHPSFC\u2010MS\/MS analysis was performed by a semi\u2010automated liquid\u2013liquid extraction method. The UHPSFC\u2010MS\/MS method used a Chiralpak AD\u20103 column with a mobile phase consisting of CO\n                    <jats:sub>2<\/jats:sub>\n                    and 0.1% ammonium hydroxide in 2\u2010propanol\/methanol (50\/50, v\/v). The injection volume was 2 \u03bcL and run time was 4 minutes. MS\/MS detection was performed with positive electrospray ionization and two multiple reaction monitoring transitions (\n                    <jats:italic>m\/z<\/jats:italic>\n                    136.1 &gt; 119.0 and\n                    <jats:italic>m\/z<\/jats:italic>\n                    136.1 &gt; 91.0). The calibration range was 12.5\u20131,000 nM for each analyte. The between\u2010assay relative standard deviations were in the range of 1.3\u20133.0%. Recovery was 73% and matrix effects ranged from 95 to 100% when corrected with internal standard. After development and validation, the method has been successfully implemented in our laboratory for both separation and quantification of R\/S\u2010amphetamine and has proved to be a reliable and useful tool for distinguishing intake of R\u2010 and S\u2010amphetamine in authentic patient samples.\n                  <\/jats:p>","DOI":"10.1002\/dta.2879","type":"journal-article","created":{"date-parts":[[2020,6,14]],"date-time":"2020-06-14T19:22:41Z","timestamp":1592162561000},"page":"1344-1353","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Enantiomeric separation and quantification of R\/S\u2010amphetamine in serum using semi\u2010automated liquid\u2010liquid extraction and ultra\u2010high performance supercritical fluid chromatography\u2010tandem mass spectrometry"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7205-8090","authenticated-orcid":false,"given":"Hilde","family":"Havnen","sequence":"first","affiliation":[{"name":"Department of Clinical Pharmacology St. Olav University Hospital  Trondheim Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5009-3802","authenticated-orcid":false,"given":"Miriam","family":"Hansen","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology St. Olav University Hospital  Trondheim Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7902-9014","authenticated-orcid":false,"given":"Olav","family":"Spigset","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology St. Olav University Hospital  Trondheim Norway"},{"name":"Department of Clinical and Molecular Medicine Norwegian University of Science and 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