{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T14:02:03Z","timestamp":1790258523368,"version":"4.1.0"},"reference-count":22,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,10,26]],"date-time":"2020-10-26T00:00:00Z","timestamp":1603670400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003693","name":"Korea Institute of Science and Technology","doi-asserted-by":"publisher","award":["an intramural grant \/ 2V08190"],"award-info":[{"award-number":["an intramural grant \/ 2V08190"]}],"id":[{"id":"10.13039\/501100003693","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human insulin\u2010like growth factor 1 (IGF\u2010I) is the primary mediator of the effects of the growth hormone (GH). Therefore, it has been used as a biomarker to detect the abuse of GH in sports. The measurement of IGF\u2010I relies on mass\u2010based and immunological approaches to analysis. Among the mass\u2010based analysis methods, liquid chromatography\u2013mass spectrometry (LC\u2013MS) has a number of functional advantages. LC\u2013MS measurements based on the quantification of IGF\u2010I, according to trypsin digestion, are used in the most common method of analyzing doping. However, this method is time\u2010consuming and subject to experimental variability.<\/jats:p><jats:p>In this study, we optimized a rapid method for detecting IGF\u2010I without the trypsin digestion step. This method of analysis uses an ultra\u2010centrifugal filter and an LC\u2010HRMS through narrow\u2010range mass scan method. To verify the validity of this method, eight categories of validation testing were applied with the following results: linearity, <jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup> &gt; 0.99; limit of detection, 15 ng\/ml; limit of quantification, 20 ng\/ml; accuracy, &gt;99%; recovery rate, &gt;95%; carryover, &lt;0.03; and inter\u2010 and intra\u2010day precision values, %CV &lt; 2% and %CV &lt; 6%, respectively. Furthermore, we discussed the correlation of the quantified concentration from two other methods, immunoradiometric assay (IRMA) and parallel reaction monitoring method, using 209 serum samples. In conclusion, although both mass spectrometry\u2010based methods worked equally well in terms of analytical performance and correlation with IRMA results, narrow\u2010range mass scan method had several advantages, such as time and cost savings and reliable reproducibility, over the existing methods.<\/jats:p>","DOI":"10.1002\/dta.2944","type":"journal-article","created":{"date-parts":[[2020,10,11]],"date-time":"2020-10-11T23:50:23Z","timestamp":1602460223000},"page":"451-459","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Optimization, validation, and comparison of a rapid method for the quantification of insulin\u2010like growth factor 1 in serum using liquid chromatography\u2013high\u2010resolution mass spectrometry"],"prefix":"10.1002","volume":"13","author":[{"given":"Yoondam","family":"Seo","sequence":"first","affiliation":[{"name":"Doping Control Center Korea Institute of Science and Technology  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jisoo","family":"Park","sequence":"additional","affiliation":[{"name":"Doping Control Center Korea Institute of Science and Technology  Seoul Republic of Korea"},{"name":"Graduate School of Pharmaceutical Sciences Ewha Womans University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minyoung","family":"Kim","sequence":"additional","affiliation":[{"name":"Doping Control Center Korea Institute of Science and Technology  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7318-7353","authenticated-orcid":false,"given":"Changmin","family":"Sung","sequence":"additional","affiliation":[{"name":"Doping Control Center Korea Institute of Science and Technology  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oh\u2010Seung","family":"Kwon","sequence":"additional","affiliation":[{"name":"Doping Control Center Korea Institute of Science and Technology  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hwa Jeong","family":"Lee","sequence":"additional","affiliation":[{"name":"Graduate School of Pharmaceutical Sciences Ewha Womans University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2791-406X","authenticated-orcid":false,"given":"Hophil","family":"Min","sequence":"additional","affiliation":[{"name":"Doping Control Center Korea Institute of Science and Technology  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,10,26]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-540-79088-4_8"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjp.2008.153"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1700013"},{"key":"e_1_2_7_5_1","first-page":"99","article-title":"Growth hormone doping: a review","volume":"2","author":"Erotokritou\u2010Mulligan I","year":"2011","journal-title":"Open Access J Sports Med"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ghir.2007.01.011"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ghir.2011.12.005"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.67.5.749"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.3388"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2013-3059"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ghir.2009.09.004"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.forsciint.2011.06.015"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-011-4859-9"},{"key":"e_1_2_7_14_1","unstructured":"Program WA\u2010D.Guidelines human growth hormone (hGH) biomarker test for doping control analysis version 2.0. In: World Anti\u2010Doping AgencyApril 2016:23."},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2013.208538"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac201800g"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.3975"},{"issue":"4","key":"e_1_2_7_18_1","first-page":"318","article-title":"Serum insulin\u2010like growth factor I quantitation by mass spectrometry: insights for protein quantitation with this technology","volume":"27","author":"Kam RK","year":"2016","journal-title":"EJIFCC"},{"key":"e_1_2_7_19_1","unstructured":"StrupatK ScheibnerO BromirskiM.High\u2010resolution accurate\u2010mass orbitrap mass spectrometry\u2014definitions opportunities and advantages. 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