{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T06:23:21Z","timestamp":1782800601698,"version":"3.54.5"},"reference-count":15,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2021,3,19]],"date-time":"2021-03-19T00:00:00Z","timestamp":1616112000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Numerous case reports of intoxications with nutmeg seeds (<jats:styled-content style=\"fixed-case\"><jats:italic>Myristica fragrans<\/jats:italic><\/jats:styled-content>, Houtt.) can be found in literature often following their abuse, as psychotropic effects were described after ingestions of large doses. The successful detection of the main ingredients of the nutmeg seeds essential oil elemicin, myristicin, and safrole, as well as their metabolites in human urine by gas chromatography coupled to mass spectrometry (GC\u2010MS) was already described. The aim of this study was to investigate the detectability of the main ingredients of nutmeg seeds and their metabolites in human blood and urine samples using liquid chromatography coupled to linear ion trap mass spectrometry (LC\u2010LIT\u2010MS<jats:sup>n<\/jats:sup>) and liquid chromatography coupled to high\u2010resolution mass spectrometry (LC\u2010HRMS\/MS) after nutmeg seed abuse. Sample material of three individuals was retrospectively investigated after a systematic screening approach indicated an intoxication with nutmeg seeds as a likely cause of symptoms. Metabolic patterns in plasma and urine using GC\u2010MS were comparable with those described in earlier publications. Investigations using hyphenated liquid chromatography techniques lead to the detection of myristicin and safrole, as well as further metabolites not described using GC\u2010MS and revealed sulfation as an additional Phase II metabolic pathway. These results might help to detect or confirm future intoxications with nutmeg seeds by using LC\u2010MS techniques.<\/jats:p>","DOI":"10.1002\/dta.3027","type":"journal-article","created":{"date-parts":[[2021,3,15]],"date-time":"2021-03-15T09:50:13Z","timestamp":1615801813000},"page":"1440-1444","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Abuse of nutmeg seeds: Detectable by means of liquid chromatography\u2010mass spectrometry techniques?"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7126-5263","authenticated-orcid":false,"given":"Sascha K.","family":"Manier","sequence":"first","affiliation":[{"name":"Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology Saarland University, Center for Molecular Signaling (PZMS)  Homburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lea","family":"Wagmann","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology Saarland University, Center for Molecular Signaling (PZMS)  Homburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Armin A.","family":"Weber","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology Saarland University, Center for Molecular Signaling (PZMS)  Homburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4377-6784","authenticated-orcid":false,"given":"Markus R.","family":"Meyer","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and Toxicology Saarland University, Center for Molecular Signaling (PZMS)  Homburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,3,19]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"586","article-title":"Nutmeg poisoning","volume":"86","author":"Green RC","year":"1918","journal-title":"Va Med Mon"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0379-0738(00)00369-8"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.3109\/15563650.2015.1079324"},{"key":"e_1_2_6_5_1","first-page":"archdischild\u201020","article-title":"The 'nutmeg challenge': a dangerous social media trend","author":"Atherton RR","year":"2020","journal-title":"Arch Dis Child"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007691-200608000-00013"},{"issue":"1","key":"e_1_2_6_7_1","first-page":"103","article-title":"Detection of nutmeg abuse by gas chromatography\u2010mass spectrometric 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