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In the literature, there have been reports of adverse events associated with the consumption of thyroid hormone\u2010containing products. In this study, an LC\u2010MS\/MS method was developed and validated for the analysis of thyroxine (T4), 3,3\u2032,5\u2010triiodo\u2010<jats:sc>l<\/jats:sc>\u2010thyronine (T3), 3,3\u2032,5\u2032\u2010triiodothyronine (rT3), 3,5\u2010diiodothyronine (3,5\u2010T2) and 3,3\u2032\u2010diiodothyronine (3,3\u2032\u2010T2) in dietary supplements. Sonication with methanol was used for the extraction of free hormones from nonglandular products. The tissue\u2010bound hormones from glandular thyroid products were extracted using a modified enzymatic digestion, in which the matrix was first extracted by sonication with methanol and then by enzymatic digestion with proteases. Both extraction methods provided acceptable recovery values between 78% and 116%. Fifty\u2010eight products making claims related to thyroid management were purchased over the internet from 2017\u20132018 and quantitatively analyzed for five hormones using the validated methods. Eleven out of 19 glandular products were found to contain quantifiable amounts of hormones. Maximum daily servings were also calculated for each product based on label information. The maximum amount of T4, T3, and rT3 per daily serving in the glandular products were up to 210, 32, and 7.6\u00a0\u03bcg\/day, respectively. In the case of nonglandular products, which were labeled to contain plant extracts, vitamins, minerals, diiodo compounds, and so forth, the amounts of 3,5\u2010T2 and 3,3\u2032\u2010T2 were up to 740 and 2700\u2009\u03bcg\/day, respectively.<\/jats:p>","DOI":"10.1002\/dta.3046","type":"journal-article","created":{"date-parts":[[2021,4,15]],"date-time":"2021-04-15T11:47:19Z","timestamp":1618487239000},"page":"578-586","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Determination of thyroid hormones in dietary supplements using liquid chromatography\u2013tandem mass spectrometry"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1523-9594","authenticated-orcid":false,"given":"Rahul S.","family":"Pawar","sequence":"first","affiliation":[{"name":"Office of Regulatory Science, Center for Food Safety and Applied Nutrition U.S. Food and Drug Administration  College Park Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satyanarayanaraju","family":"Sagi","sequence":"additional","affiliation":[{"name":"Office of Regulatory Science, Center for Food Safety and Applied Nutrition U.S. Food and Drug Administration  College Park Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dmitry","family":"Leontyev","sequence":"additional","affiliation":[{"name":"Office of Regulatory Science, Center for Food Safety and Applied Nutrition U.S. Food and Drug Administration  College Park Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,5,18]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201601116"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.1.06131"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem.87.2.8182"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/17512433.2016.1182019"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12020-019-02023-7"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.2013-1083"},{"issue":"6","key":"e_1_2_6_8_1","first-page":"1375","article-title":"A thyrotoxicosis outbreak due to dietary pills in Paris","volume":"4","author":"Ioos V","year":"2008","journal-title":"Ther Clin Risk Manag"},{"key":"e_1_2_6_9_1","doi-asserted-by":"crossref","unstructured":"ReginaA MajlesiN.Thyrotoxicosis after consumption of dietary supplements purchased through the internet\u2014Staten Island New York 2015. 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