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Twenty\u2010five milligrams of hair were incubated with 500\u2010\u03bcl M3\u00ae buffer reagent at 100\u00b0C for 1\u2009h for complete digestion. After cooling, 1\u2010\u03bcl supernatant was injected onto chromatography system. Urine samples were simply diluted before injection. The chromatographic run time was short (8\u2009min) through a column with a mobile phase gradient. The method was linear (determination coefficients always higher than 0.99) from limit of quantification (LOQ) to 500\u2009ng\/ml in urine and from LOQ to 10\u2009ng\/mg in hair. LOQs ranged from 0.07 to 1.16\u2009ng\/ml in urine and from 0.02 to 0.15\u2009ng\/mg in hair. No significant ion suppression due to matrix effect was observed, and process efficiency was always higher than 80%. Intra\u2010 and inter\u2010assay precision was lower than 15%. The suitability of the methods was tested with six urine and hair specimens from patients treated with acetazolamide, dorzolamide, or brinzolamide for ocular diseases or systemic hypertension. Average urine concentrations were 266.32\u2009ng\/ml for dorzolamide and 47.61\u2009ng\/ml for <jats:italic>N<\/jats:italic>\u2010deethyl\u2010dorzolamide (<jats:italic>n<\/jats:italic>\u2009=\u20093), 109.27\u2009ng\/ml for brinzolamide and 1.02\u2009ng\/ml for <jats:italic>O<\/jats:italic>\u2010desmethyl\u2010brinzolamide (<jats:italic>n<\/jats:italic>\u2009=\u20092), and finally, 12.63\u2009ng\/ml for acetazolamide. Average hair concentrations were 5.94\u2009ng\/mg for dorzolamide and 0.048\u2009ng\/mg for <jats:italic>N<\/jats:italic>\u2010deethyl\u2010dorzolamide (<jats:italic>n<\/jats:italic>\u2009=\u20093), 3.26\u2009ng\/mg for brinzolamide (<jats:italic>n<\/jats:italic>\u2009=\u20092), and 2.3\u2009ng\/mg for acetazolamide (<jats:italic>n<\/jats:italic>\u2009=\u20091). The developed method was simple and fast both in the extraction procedures making it eligible in high\u2010throughput analysis for clinical forensic and doping purposes.<\/jats:p>","DOI":"10.1002\/dta.3055","type":"journal-article","created":{"date-parts":[[2021,4,27]],"date-time":"2021-04-27T23:56:49Z","timestamp":1619567809000},"page":"1552-1560","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Development and validation of a fast ultra\u2010high\u2010performance liquid chromatography tandem mass spectrometry method for determining carbonic anhydrase inhibitors and their metabolites in urine and hair"],"prefix":"10.1002","volume":"13","author":[{"given":"Alfredo Fabrizio","family":"Lo Faro","sequence":"first","affiliation":[{"name":"Department of Excellence of Biomedical Sciences and Public Health University \u201cPolitecnica delle Marche\u201d of Ancona  Ancona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7814-8038","authenticated-orcid":false,"given":"Anastasio","family":"Tini","sequence":"additional","affiliation":[{"name":"Department of Excellence of Biomedical Sciences and Public Health University \u201cPolitecnica delle Marche\u201d of Ancona  Ancona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massimo","family":"Gottardi","sequence":"additional","affiliation":[{"name":"Comedical s.r.l.  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