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The most challenging compounds among this class are the endogenous anabolic steroids, which are detected by quantitative measurement of testosterone (T) and its metabolites with a so\u2010called \u201csteroid profiling\u201d method. The current steroid profile is based on the concentrations and ratios of the sum of free and glucuronidated steroids. Recently, our group developed a steroid profiling method for the detection of three free steroids and 14 intact steroid conjugates, including both the glucuronic acid conjugated and sulfated fraction. The study aimed at evaluating the long\u2010term stability of steroid conjugate concentrations and ratios, and the influence of different endogenous steroids on this extended steroid profile. A single dose of oral T undecanoate (TU), topical T gel, topical dihydrotestosterone (DHT) gel, and oral dehydroepiandrosterone (DHEA) was administered to six healthy male volunteers. One additional volunteer with a homozygote deletion of the UGT2B17 gene (del\/del genotype) received a single topical dose of T gel. An intramuscular dose of TU was administered to another volunteer. To avoid fluctuation of steroid concentrations caused by variations in urinary flow rates, steroid ratios were calculated and evaluated as possible biomarkers for the detection of endogenous steroid abuse with low doses. Overall, sulfates do not have substantial additional value in prolonging detection times for the investigated endogenous steroids and administration doses. The already monitored glucuronides were overall the best markers and were sufficient to detect the administered steroids.<\/jats:p>","DOI":"10.1002\/dta.3096","type":"journal-article","created":{"date-parts":[[2021,5,13]],"date-time":"2021-05-13T00:21:38Z","timestamp":1620865298000},"page":"851-863","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Long\u2010term stability study and evaluation of intact steroid conjugate ratios after the administration of endogenous steroids"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3472-2170","authenticated-orcid":false,"given":"Laurie","family":"De Wilde","sequence":"first","affiliation":[{"name":"Department Diagnostic Sciences, Doping Control Laboratory (DoCoLab) Ghent University (UGent)  Ghent Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pieter","family":"Van Renterghem","sequence":"additional","affiliation":[{"name":"Department Diagnostic Sciences, Doping Control Laboratory (DoCoLab) Ghent University (UGent)  Ghent Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Van Eenoo","sequence":"additional","affiliation":[{"name":"Department Diagnostic Sciences, Doping Control Laboratory (DoCoLab) Ghent University (UGent)  Ghent Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,5,25]]},"reference":[{"key":"e_1_2_5_2_1","unstructured":"The World Anti\u2010Doping Agency.2018 Anti\u2010Doping Testing Figures;2018."},{"key":"e_1_2_5_3_1","first-page":"1","article-title":"Direct quantitation of endogenous steroid sulfates in human urine by liquid chromatography\u2010electrospray tandem mass spectrometry","author":"Esquivel A","year":"2018","journal-title":"Drug Test 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