{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T18:17:18Z","timestamp":1790965038308,"version":"4.1.0"},"reference-count":20,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,7,3]],"date-time":"2021-07-03T00:00:00Z","timestamp":1625270400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100013934","name":"Bundesministerium des Innern, f\u00fcr Bau und Heimat","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013934","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In order to detect the misuse of testosterone (T) or boldenone (Bo) in doping control analysis, the confirmation of atypical findings employing the determination of carbon isotope ratios (CIR) is mandatory for issuing adverse analytical findings. Elevated concentrations of T (or elevated T\/epitestosterone ratios) may result from confounding factors such as ethanol intake, and the presence of low urinary concentrations of Bo can originate from endogenous or urinary in situ production of small amounts of the steroid. As pharmaceutical preparations of Bo and T are generally depleted in <jats:sup>13<\/jats:sup>C, their CIR differ significantly from the <jats:sup>13<\/jats:sup>C\u2010enriched endogenous steroids. Some rare cases have been reported on pharmaceutical preparations showing <jats:sup>13<\/jats:sup>C\u2010enriched isotope ratios that complicate the current application of CIR in sports drug testing. Therefore, the CIR of a subset of <jats:italic>n<\/jats:italic>\u2009=\u2009157 T preparations and <jats:italic>n<\/jats:italic>\u2009=\u200939 Bo preparations seized in Switzerland and Germany between 2013 and 2018 was analyzed in order to estimate the possible impact of steroid preparations showing <jats:sup>13<\/jats:sup>C\u2010enriched isotope ratios on the current approach to detect their misuse. All investigated Bo preparations showed CIR in the expected range between \u2013 26.7 and \u221230.3\u2030. Within the T samples, 95% showed the expected values below \u221226\u2030 while six samples fall between \u221225 and \u221226\u2030 and one sample was indistinguishable from endogenously produced T with a CIR of \u221223.3\u2030.<\/jats:p>","DOI":"10.1002\/dta.3120","type":"journal-article","created":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T05:58:13Z","timestamp":1625119093000},"page":"514-518","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Investigations in carbon isotope ratios of seized testosterone and boldenone preparations"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7462-6693","authenticated-orcid":false,"given":"Thomas","family":"Piper","sequence":"first","affiliation":[{"name":"German Sport University Cologne Center for Preventive Doping Research \u2013 Institute of Biochemistry  K\u00f6ln Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1535-6451","authenticated-orcid":false,"given":"Mario","family":"Thevis","sequence":"additional","affiliation":[{"name":"German Sport University Cologne Center for Preventive Doping Research \u2013 Institute of Biochemistry  K\u00f6ln Germany"},{"name":"European Monitoring Center for Emerging Doping Agents (EuMoCEDA)  Cologne\/Bonn Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,7,3]]},"reference":[{"key":"e_1_2_6_2_1","unstructured":"World Anti\u2010Doping Code International Standard Prohibited List 2021.https:\/\/www.wada-ama.org\/sites\/default\/files\/resources\/files\/2021list_en.pdf accessed 02.06.2021"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jms.1457"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.396"},{"key":"e_1_2_6_5_1","first-page":"211","volume-title":"Recent Advances in Doping Analysis (2)","author":"Sch\u00e4nzer W","year":"1995"},{"key":"e_1_2_6_6_1","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1002\/dta.124","article-title":"Determination of 13C\/12C ratios of urinary excreted boldenone and its main metabolite 5\u03b2\u2010androst\u20101\u2010en\u201017\u03b2\u2010ol\u20103\u2010one","volume":"2","author":"Piper T","year":"2010","journal-title":"Drug Test Anal"},{"key":"e_1_2_6_7_1","unstructured":"WADA Technical Document \u2013 TD2021IRMS.WADA Science\/IRMS Working Group.https:\/\/www.wada-ama.org\/sites\/default\/files\/resources\/files\/td2021irms_final_eng.pdf accessed 02.06.2021"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.5129"},{"key":"e_1_2_6_9_1","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1002\/dta.15","article-title":"13C\/12C ratios of endogenous urinary steroids investigated for doping control purposes","volume":"1","author":"Piper T","year":"2009","journal-title":"Drug Test Anal"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0039-128X(96)00253-X"},{"key":"e_1_2_6_11_1","first-page":"275","volume-title":"Recent Advances in Doping Analysis (4)","author":"Horning S","year":"1996"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0731-7085(00)00452-0"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.175"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.1533"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.373"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.1745"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.steroids.2008.10.001"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.forsciint.2015.10.001"},{"key":"e_1_2_6_19_1","unstructured":"PiperT HaeneltN Fussh\u00f6llerG GeyerH ThevisM.Sensitive detection of testosterone and testosterone prohormone administrations based on urinary concentrations and carbon isotope ratios of androsterone and etiocholanolone. in preparation"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.2530"},{"key":"e_1_2_6_21_1","unstructured":"HullsteinI YuQ DehnesY.Carbon isotope ratio determination of seized nandrolone preparations in comparison to results from analyses of 19\u2010norandrosterone in urine samples. Presented at the MANFRED DONIKE WORKSHOP2021."}],"container-title":["Drug Testing and Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dta.3120","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/dta.3120","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dta.3120","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T09:43:50Z","timestamp":1760348630000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/dta.3120"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7,3]]},"references-count":20,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2022,3]]}},"alternative-id":["10.1002\/dta.3120"],"URL":"https:\/\/doi.org\/10.1002\/dta.3120","archive":["Portico"],"relation":{},"ISSN":["1942-7603","1942-7611"],"issn-type":[{"value":"1942-7603","type":"print"},{"value":"1942-7611","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,7,3]]},"assertion":[{"value":"2021-06-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-06-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-07-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}