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The aim of the present work is the development and validation of a targeted liquid chromatography tandem mass spectrometry (LC\u2010MS\/MS) method for the detection of NPS in whole blood.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>A protein\u2010precipitation based LC\u2010MS\/MS method for the detection of more than 180 NPS was developed and validated by assessing the following parameters: selectivity, linearity, accuracy, precision, limit of detection (LOD) and of quantification (LOQ) recovery, and matrix effect. Then, the method was applied to real forensic samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The method allowed the identification of 132 synthetic cannabinoids, 22 synthetic opioids, and 28 substances among synthetic cathinones, stimulants, and other drugs. Validation was successfully achieved for most of the compounds. Linearity was in the range of 0.25\u201310\u00a0ng\/ml for synthetic cannabinoids and 0.25\u201325\u2009ng\/ml for other drugs. Accuracy and precision were acceptable according to international guidelines. Three cases tested positive for fentanyl and ketamine, in the setting of emergency room administration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The present methodology represents a fast, not expensive, wide\u2010panel method for the analysis of more than 180 NPS by LC\u2010MS\/MS, which can be profitably applied both in a clinical context and in postmortem toxicology.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dta.3170","type":"journal-article","created":{"date-parts":[[2021,10,4]],"date-time":"2021-10-04T06:51:41Z","timestamp":1633330301000},"page":"202-223","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["Development and validation of a rapid LC\u2010MS\/MS method for the detection of 182 novel psychoactive substances in whole blood"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0441-9787","authenticated-orcid":false,"given":"Arianna","family":"Giorgetti","sequence":"first","affiliation":[{"name":"Department of Medical and Surgical Sciences, Unit of Legal Medicine University of Bologna  Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rossella","family":"Barone","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences, Unit of Legal Medicine University of Bologna  Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guido","family":"Pelletti","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences, Unit of Legal Medicine University of Bologna  Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Garagnani","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences, Unit of Legal Medicine University of Bologna  Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Pascali","sequence":"additional","affiliation":[{"name":"Department of Legal and Occupational Medicine, Toxicology and Public Health University Hospital of Padova  Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2954-7539","authenticated-orcid":false,"given":"Belal","family":"Haschimi","sequence":"additional","affiliation":[{"name":"Institute of Forensic Medicine, Forensic Toxicology, Medical Center, Faculty of Medicine University of Freiburg  Freiburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1883-2804","authenticated-orcid":false,"given":"Volker","family":"Auw\u00e4rter","sequence":"additional","affiliation":[{"name":"Institute of Forensic Medicine, Forensic Toxicology, Medical Center, Faculty of Medicine University of Freiburg  Freiburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,10,21]]},"reference":[{"key":"e_1_2_7_2_1","unstructured":"EMCDDA.European drug report. 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