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Other POCTs identify MDMA in sweat by detecting the drug as a cross\u2010reactant rather than target analyte, thus decreasing the test's sensitivity. The study's aim was to design a sensitive POCT for the detection of MDMA in LFPs using surface plasmon resonance (SPR) and lateral flow immunoassay (LFA) technology. A high\u2010affinity antibody binding pair was identified using the former technique, deeming the pair suitable for a LFA. Titrations of fluorescently labelled antibody and antigen concentrations were tested to identify a sharp drop\u2010in signal upon the addition of MDMA to allow a clear distinction between negative and positive outcomes. We trialled the LFA by producing dose response curves with MDMA and a group of drugs that share a similar chemical structure to MDMA. These were generated through spiking the LFA with increasing levels of drug (0\u2013400\u2009pg\/10\u00a0\u03bcl of MDMA; 0\u201310,000\u2009pg\/10\u00a0\u03bcl of cross\u2010reactant). Fluorescent test signals were measured using a cartridge reader. The cut\u2010off (threshold) 60\u2009pg\/10\u00a0\u03bcl calculated better cartridge performance (1.00 sensitivity, 0.95 specificity and 0.98 accuracy), when compared with 40\u2009pg\/10\u00a0\u03bcl. The biggest cross\u2010reactant was PMMA (250%), followed by MDEA (183%), MBDB (167%), MDA (16%) and methamphetamine (16%). A sensitive LFP screening tool requiring no sample preparation was successfully designed.<\/jats:p>","DOI":"10.1002\/dta.3196","type":"journal-article","created":{"date-parts":[[2021,11,11]],"date-time":"2021-11-11T23:45:03Z","timestamp":1636674303000},"page":"613-621","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Development of a point\u2010of\u2010care test for the detection of MDMA in latent fingerprints using surface plasmon resonance and lateral flow technology"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6994-7815","authenticated-orcid":false,"given":"Caroline","family":"Pollard","sequence":"first","affiliation":[{"name":"King's Forensics, Department of Analytical, Environmental &amp; Forensic Sciences, Franklin\u2010Wilkins Building King's College London  150 Stamford St London United Kingdom SE1 9NH UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Hudson","sequence":"additional","affiliation":[{"name":"Intelligent Fingerprinting  Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James M.","family":"McDonnell","sequence":"additional","affiliation":[{"name":"Randall Centre for Cell and Molecular Biophysics King's College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7101-2776","authenticated-orcid":false,"given":"Paul G.","family":"Royall","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Science, Franklin\u2010Wilkins Building King's College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kim","family":"Wolff","sequence":"additional","affiliation":[{"name":"King's Forensics, Department of Analytical, Environmental &amp; Forensic Sciences, Franklin\u2010Wilkins Building King's College London  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