{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T19:50:31Z","timestamp":1770839431527,"version":"3.50.1"},"reference-count":13,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,11,21]],"date-time":"2021-11-21T00:00:00Z","timestamp":1637452800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100013935","name":"Partnership for Clean Competition","doi-asserted-by":"publisher","award":["2019 R219"],"award-info":[{"award-number":["2019 R219"]}],"id":[{"id":"10.13039\/100013935","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The World Anti\u2010Doping Agency (WADA) prohibits athletes from using recombinant growth hormone (GH). The validated method used in antidoping laboratories for the direct detection of exogenous GH in serum requires two immunoluminometric assays (ILMAs): The first mainly measures the concentration of the full\u2010length (22\u2009kDa) form of GH (recGH), and the second measures concentrations of multiple GH fragments produced by the pituitary gland (22\u2009kDa, 20\u2009kDa and other forms) (pitGH). The tube\u2010by\u2010tube analysis is laborious. A recent development opened new possibilities to simplify the detection of recGH in serum: multiplexed immunoassays that detect multiple targets in a single well of a 96\u2010well plate using an ELISA\u2010like procedure with high sensitivity.<\/jats:p><jats:p>Our aim was to evaluate this technology by developing a customized assay for GH detection. One pair of antibodies with specificities similar to those of the recGH assay and one pair of antibodies compatible with pitGH detection were selected for a single duplex assay. Forty\u2010eight serum samples (negative athlete samples and positive samples following GH administration) were analyzed using the two methods. The microplate duplex assay discriminated between the negative athlete samples and the positive controls, although the rec\/pit ratios from the duplex assay were lower than those obtained with the ILMAs. This new assay would offer a modern alternative to ILMAs, with fewer analytical steps and a smaller sample volume. However, an adaptation of the decision limits seems mandatory.<\/jats:p>","DOI":"10.1002\/dta.3197","type":"journal-article","created":{"date-parts":[[2021,11,11]],"date-time":"2021-11-11T02:06:19Z","timestamp":1636596379000},"page":"724-732","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Development of a microplate duplex immunoassay to simplify detection of growth hormone doping: Proof of concept"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2670-6939","authenticated-orcid":false,"given":"Alexandre","family":"Marchand","sequence":"first","affiliation":[{"name":"D\u00e9partement des Analyses Agence Fran\u00e7aise de Lutte contre le Dopage (AFLD)  Ch\u00e2tenay\u2010Malabry France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daisy","family":"Roy","sequence":"additional","affiliation":[{"name":"Meso Scale Diagnostics LLC  Rockville Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah A.","family":"Monsheimer","sequence":"additional","affiliation":[{"name":"Meso Scale Diagnostics LLC  Rockville Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer","family":"Lewis","sequence":"additional","affiliation":[{"name":"Meso Scale Diagnostics LLC  Rockville Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magnus","family":"Ericsson","sequence":"additional","affiliation":[{"name":"D\u00e9partement des Analyses Agence Fran\u00e7aise de Lutte contre le Dopage (AFLD)  Ch\u00e2tenay\u2010Malabry France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,11,21]]},"reference":[{"key":"e_1_2_9_2_1","unstructured":"TD2021GH.Human Growth Hormone (hGH) isoform differential immunoassays for doping control analyses v1.0. WADA Technical Document2021."},{"key":"e_1_2_9_3_1","unstructured":"Guidelines Human Growth Hormone Biomarkers Test.Human Growth Hormone (hGH) Biomarkers test for doping control analyses v. 3.0. WADA Guidelines2021."},{"key":"e_1_2_9_4_1","first-page":"99","article-title":"Growth hormone doping: a review","volume":"2","author":"Erotokritou\u2010Mulligan I","year":"2011","journal-title":"Open Access J Sports Med"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ghir.2013.01.002"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3155"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.2506"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.2674"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-0223-2_6"},{"key":"e_1_2_9_10_1","unstructured":"MSD.MULTI\u2010ARRAY technology. 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