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To study the prevalence of NSAIDs intake in ultratrail runners, oral fluid (OF) is a relevant matrix as it is noninvasive and easy to collect. The aim of our work was to develop and validate a liquid\u2013liquid extraction followed by a liquid chromatography (LC)\u2013mass spectrometry (MS)\/high resolution mass spectrometry (HRMS) method for the simultaneous quantification of 19 NSAIDs in OF. After a comparison of different liquid\u2013liquid extraction methods, a double step liquid\u2013liquid extraction with chloroform was performed on OF collected with Quantisal\u00ae, with extraction recoveries higher than 90%. An Accucore AQ column was selected for the chromatographic separation of NSAIDs. The Q Exactive Plus mass spectrometer operated in full scan and ddms2 mode after positive and negative electrospray ionization. Selectivity, carry\u2010over, matrix effect, and linearity were validated for all NSAIDs. Within\u2010day and between\u2010day accuracy and precision were validated for all NSAIDs (&lt;15% for quality control [QC] samples and &lt;20% for lower limit of quantitation [LLOQ]), except within\u2010day accuracy for the LLOQ of mefenamic acid. A stability study was also performed on OF at room temperature and +4\u00b0C. The method was applied on OF from runners who participate to Ultra Trail du Mont Blanc\u00ae.<\/jats:p>","DOI":"10.1002\/dta.3216","type":"journal-article","created":{"date-parts":[[2022,1,5]],"date-time":"2022-01-05T21:29:16Z","timestamp":1641418156000},"page":"701-712","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Simultaneous quantification of 19 nonsteroidal anti\u2010inflammatory drugs in oral fluid by liquid chromatography\u2010high resolution mass spectrometry: Application on ultratrail runner's oral fluid"],"prefix":"10.1002","volume":"14","author":[{"given":"Mohammad Shafiq","family":"Mashal","sequence":"first","affiliation":[{"name":"Biochemistry and Pharmaco\u2010toxicology laboratory, Lyon Sud Hospital University Hospital of Lyon  Pierre\u2010B\u00e9nite France"},{"name":"Toxicology Department, Pharmacy Faculty Kabul University  Kabul 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