{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T06:15:54Z","timestamp":1786947354715,"version":"build-2736575974"},"reference-count":33,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T00:00:00Z","timestamp":1648771200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In Taiwan, synthetic cathinones are the most prevalent new psychoactive substances, and their use is growing continuously. Urine samples are currently analysed to determine drug abuse, but the metabolic profiles and metabolites of these compounds are not widely reported. Given that cases of eutylone abuse have been growing since 2020, this study established a method employing supported liquid extraction combined with liquid chromatography tandem quadrupole time\u2010of\u2010flight mass spectrometry to identify and quantify eutylone and its metabolites in urine samples. Method validation was performed, and eight authentic samples were analysed. Moreover, <jats:italic>in vitro<\/jats:italic> metabolism experiments were conducted, and metabolites were generated by incubating eutylone with human liver microsomes and cytosol. Metabolite characterisation was achieved by confirming the accurate mass and product ions in full MS\/MS spectra. Five metabolites were identified in <jats:italic>in vitro<\/jats:italic> experiments; they resulted from eutylone N\u2010dealkylation, \u03b2\u2010ketone reduction, demethylenation, aliphatic hydroxylation and sequential demethylenation and O\u2010methylation. The metabolic profile was obtained evaluating the metabolites at different incubation times: Demethylenation occurred first, followed by N\u2010dealkylation, \u03b2\u2010ketone reduction and aliphatic hydroxylation. Three additional metabolites were identified in authentic samples. Based on <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> evidence, we propose that the demethylenation and O\u2010methylation metabolite, the \u03b2\u2010ketone reduction metabolite, and the \u03b2\u2010ketone reduction, demethylenation and O\u2010methylation metabolite are the most appropriate biomarkers of eutylone consumption. Using these markers can help expand the eutylone detection window and provide information for toxicology research.<\/jats:p>","DOI":"10.1002\/dta.3258","type":"journal-article","created":{"date-parts":[[2022,3,24]],"date-time":"2022-03-24T20:12:55Z","timestamp":1648152775000},"page":"1325-1337","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Quantitative determination and metabolic profiling of synthetic cathinone eutylone <i>in vitro<\/i> and in urine samples by liquid chromatography tandem quadrupole time\u2010of\u2010flight mass spectrometry"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3765-9189","authenticated-orcid":false,"given":"Ya\u2010Ling","family":"Yeh","sequence":"first","affiliation":[{"name":"Department of Forensic Science Central Police University  Taoyuan Taiwan"},{"name":"Forensic Science Section Hsinchu City Police Bureau  Hsinchu Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8498-5358","authenticated-orcid":false,"given":"Sheng\u2010Meng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Forensic Science Central Police University  Taoyuan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,4]]},"reference":[{"key":"e_1_2_7_2_1","unstructured":"United Nations Office on Drugs and CrimeWorld drug report 2021.https:\/\/www.unodc.org\/unodc\/en\/data-and-analysis\/wdr2021.html. Accessed September 21 2021."},{"key":"e_1_2_7_3_1","unstructured":"United Nations Office on Drugs and Crime.Current NPS threats volume III.https:\/\/www.drugsandalcohol.ie\/33263\/1\/Current_NPS_Threats_Vol.3.pdf. Accessed September 21 2021."},{"key":"e_1_2_7_4_1","unstructured":"United Nations Office on Drugs and Crime.Early warning advisory (EWA) on new psychoactive substances (NPS).https:\/\/www.unodc.org\/LSS\/Home\/NPS. Accessed September 21 2021."},{"key":"e_1_2_7_5_1","unstructured":"Taiwan Ministry of Health and Welfare.List of new psychoactive substances (NPS) detected in Taiwan.https:\/\/www.fda.gov.tw\/TC\/site.aspx?sid=9958&r=155488991. 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