{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:39:58Z","timestamp":1786977598188,"version":"build-2736575974"},"reference-count":33,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2022,5,20]],"date-time":"2022-05-20T00:00:00Z","timestamp":1653004800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Human oral fluid is well established as a matrix for drug screening, particularly in the workplace. The need to synthesise synthetic oral fluid (SOF) has been recognised in order to overcome human oral fluid's composition variability. We have used SOF spiked with six common drugs of abuse or their primary metabolites: morphine, amfetamine, benzoylecgonine, cocaine, diazepam, and (\u2212)\u2010\u0394\n                    <jats:sup>9<\/jats:sup>\n                    \u2010tetrahydrocannabinol (THC) in order to assess the suitability of this matrix for quality assurance purposes. For confirmation of a drug screening test, controls and spiked standards are normally required. All our analytes were detected by LC\u2013MS\/MS using a quick and easy \u201cdilute and inject\u201d sample preparation approach as opposed to relatively slower solid\u2010phase extraction. The limit of detection (LOD) was 10\u00a0ng\/ml for diazepam and THC and 5 ng\/ml for morphine, amfetamine, benzoylecgonine and cocaine. Validation results showed good accuracy as well as inter\u2010 and intra\u2010assay precision (CV [%]\u2009&lt;\u20095). Our work highlighted the importance of adding Tween\u00ae 20 to the SOF and calibrants to reduce losses when handling THC. Furthermore, drug stability was tested at various temperatures (5\u00b0C, 20\u00b0C and 40\u00b0C), for a number of days or after freeze\u2013thaw cycles. Recommendations regarding storage are provided, the spiked SOF being stable at 5\u00b0C for up to 1\u00a0week without significant drug concentration loss.\n                  <\/jats:p>","DOI":"10.1002\/dta.3279","type":"journal-article","created":{"date-parts":[[2022,5,6]],"date-time":"2022-05-06T04:04:18Z","timestamp":1651809858000},"page":"1482-1490","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Stability of drugs of abuse in synthetic oral fluid investigated using a simple \u201cdilute and inject\u201d method of analysis"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4942-6398","authenticated-orcid":false,"given":"Ivana","family":"Gavrilovi\u0107","sequence":"first","affiliation":[{"name":"Drug Control Centre, King's Forensics King's College London  London"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Musenga","sequence":"additional","affiliation":[{"name":"Drug Control Centre, King's Forensics King's College London  London"},{"name":"Laboratoire Suisse d'Analyse du Dopage Centre Hospitalier Universitaire Vaudois et Universit\u00e9 de Lausanne  Epalinges Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kim","family":"Wolff","sequence":"additional","affiliation":[{"name":"Drug Control Centre, King's Forensics King's College London  London"},{"name":"Department of Analytical, Environmental and Forensic Sciences King's College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alison","family":"Woffendin","sequence":"additional","affiliation":[{"name":"Drug Control Centre, King's Forensics King's College London  London"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Smart","sequence":"additional","affiliation":[{"name":"Drug Control Centre, King's Forensics King's College London  London"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Gong","sequence":"additional","affiliation":[{"name":"Home Office Centre for Applied Science and Technology (CAST)  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duncan","family":"Harding","sequence":"additional","affiliation":[{"name":"Home Office Centre for Applied Science and Technology (CAST)  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6407-1113","authenticated-orcid":false,"given":"David","family":"Cowan","sequence":"additional","affiliation":[{"name":"Department of Analytical, Environmental and Forensic Sciences King's College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,5,20]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1067\/mpr.2001.113778"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bdj.4807861"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345870660S103"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdent.2004.10.009"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.5005\/jcdp\u20109\u20103\u201072"},{"key":"e_1_2_7_7_1","first-page":"1","volume-title":"Saliva and The Control of its Secretion","author":"Ekstr\u00f6m J"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac050161r"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12116"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oooo.2016.03.002"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.2190\/PM.48.3.d"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12936\u2010017\u20102151\u2010y"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2014.08.037"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2008.108670"},{"key":"e_1_2_7_15_1","unstructured":"WolffK AgombarR ClatworthyA et al.Expert panel on drug driving: alternative matrices for confirmatory testing.2017. 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