{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T06:14:50Z","timestamp":1786947290396,"version":"build-2736575974"},"reference-count":35,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100017190","name":"Anti Doping Danmark","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100017190","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Dried blood spot (DBS) testing allows fast, easy and minimally invasive collection of microvolumes of blood. In an anti\u2010doping context, DBS testing has particular relevance for substances prohibited in\u2010competition only such as ephedrine, which is currently detected by urine analysis, because DBS can add information about the blood drug concentrations during the in\u2010competition period. Several collection methods and devices exist for DBS collection from different anatomical sites. Thus, agreements between concentrations of target analytes in DBS samples from different sampling sites, along with between DBS and those in conventional venous plasma samples, need to be evaluated. Herein, we collected matched upper\u2010arm DBS, fingerprick DBS and venous plasma samples from eight healthy male subjects in an 8\u2010h period following oral administrations of 20\u2009mg (\u2018low dose\u2019) and 60\u2009mg (\u2018high dose\u2019) of ephedrine. We show that the use of alternative sampling sites and matrices is a feasible possibility for ephedrine analysis in doping control. We observed very good agreement between collection sites and that specificity and sensitivity can be upheld despite use of an alternative collection site. However, potential concentration differences between DBS and venous plasma should be considered, and distinct threshold might be necessary if implementing both blood matrices in ephedrine analysis.<\/jats:p>","DOI":"10.1002\/dta.3338","type":"journal-article","created":{"date-parts":[[2022,6,23]],"date-time":"2022-06-23T21:03:36Z","timestamp":1656018216000},"page":"1685-1695","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Analysis of dried blood spots is a feasible alternative for detecting ephedrine in doping control"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4589-1711","authenticated-orcid":false,"given":"Sara Amalie","family":"Solheim","sequence":"first","affiliation":[{"name":"Science and Research Anti Doping Denmark  Br\u00f8ndby Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1199-0743","authenticated-orcid":false,"given":"Andreas","family":"Thomas","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry\/Center for Preventive Doping Research German Sport University Cologne  Cologne Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas Kamm","family":"Ringsted","sequence":"additional","affiliation":[{"name":"Science and Research Anti Doping Denmark  Br\u00f8ndby Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Thevis","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry\/Center for Preventive Doping Research German Sport University Cologne  Cologne Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Breenfeldt Andersen","sequence":"additional","affiliation":[{"name":"Science and Research Anti Doping Denmark  Br\u00f8ndby Denmark"},{"name":"Department of Nutrition, Exercise and Sports University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrik","family":"Holm\u2010S\u00f8rensen","sequence":"additional","affiliation":[{"name":"Department of Nutrition, Exercise and Sports University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7077-9265","authenticated-orcid":false,"given":"Nikolai B.","family":"Nordsborg","sequence":"additional","affiliation":[{"name":"Department of Nutrition, Exercise and Sports University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jakob","family":"M\u00f8rkeberg","sequence":"additional","affiliation":[{"name":"Science and Research Anti Doping Denmark  Br\u00f8ndby Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,7]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2016.03.055"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.4155\/bio\u20102021\u20100019"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.1803"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.342"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1039\/c5ay01514f"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.2790"},{"key":"e_1_2_8_8_1","unstructured":"World Anti\u2010Doping Agency.The 2021 prohibited list. Montreal Quebec;2021.https:\/\/www.wada-ama.org\/sites\/default\/files\/resources\/files\/2021list_en.pdf"},{"key":"e_1_2_8_9_1","volume-title":"Ephedrine","author":"Statler AK","year":"2021"},{"key":"e_1_2_8_10_1","unstructured":"WADA Science\/DL Working Group.WADA technical document\u2010TD2021DL: decision limits for the confirmatory quantification of exogenous threshold substances by chromatography\u2010based analytical methods. 1.0. Montreal Quebec: World Anti Doping Agency;2021.https:\/\/www.wada-ama.org\/sites\/default\/files\/resources\/files\/td2021dl_final_eng.pdf"},{"key":"e_1_2_8_11_1","unstructured":"WADA.The 2022 prohibited list. Montreal Quebec: World Anti\u2010Doping Agency;2022.https:\/\/www.wada-ama.org\/sites\/default\/files\/resources\/files\/2022list_final_en.pdf"},{"key":"e_1_2_8_12_1","unstructured":"WADA.International standard for testing and investigations (ISTI). Montreal Quebec: WADA;2021.https:\/\/www.wada-ama.org\/sites\/default\/files\/resources\/files\/international_standard_isti_-_2021.pdf"},{"key":"e_1_2_8_13_1","unstructured":"Ephedrine hydrochloride 15mg tablets. The Electronic Medicines Compendium.https:\/\/www.medicines.org.uk\/emc\/product\/2577\/smpc. Published2017. Accessed March 18 2020."},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005768\u2010200202000\u201000024"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1249\/01.MSS.0000069916.49903.70"},{"key":"e_1_2_8_16_1","unstructured":"ASHP.Ephedrine. Drug.com.https:\/\/www.drugs.com\/monograph\/ephedrine.html. 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