{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T15:00:07Z","timestamp":1774537207521,"version":"3.50.1"},"reference-count":20,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2022,8,14]],"date-time":"2022-08-14T00:00:00Z","timestamp":1660435200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>A young male was found dead on the bed of a hotel room. He was expected to take part in a bodybuilding competition the day after. During the site inspection, drugs of different types were found. The next day, an autopsy was performed. The evidence of cardiomegaly with organ congestion involving lung, liver, kidneys, adrenal glands, spleen and brain was confirmed by both the autoptic and the histopathological exam. However, the cause of death needed to be investigated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A thorough toxicological investigation was undertaken by gas chromatography\u2013mass spectrometry (GC\u2010MS), liquid chromatography\u2010high resolution mass spectrometry (LC\u2010HRMS) and liquid chromatography\u2013tandem mass spectrometry (UPLC\u2010MS\/MS) on samples of urine, blood and hair.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results and discussion<\/jats:title><jats:p>Clenbuterol, a long\u2010acting selective beta<jats:sub>2<\/jats:sub> agonist, was found in both blood (1\u00a0ng\/ml) and urine (1\u00a0ng\/ml), and evidence of its use was provided by the analysis of the 3\u2010cm hair (25\u2009pg\/mg). The main metabolite of drostanolone (2 alpha\u2010methyl\u2010androsterone), an anabolic steroid, was found in the urine (202\u2009ng\/ml), where an increased ratio of testosterone\/epitestosterone (T\/E\u2009=\u200911) emerged. Due to the results of the hair analysis, a long\u2010term use of various anabolic steroids was supposed. The integrated analysis of the results and the absence of other possible causes (such as trauma or cardiac conduction anomalies) led to the identification of the abuse of doping substances as the underlying cause of death.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Hair analysis has proven to be crucial in identifying drug misuse and the contributing cause of death.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dta.3350","type":"journal-article","created":{"date-parts":[[2022,8,14]],"date-time":"2022-08-14T20:44:10Z","timestamp":1660509850000},"page":"1795-1799","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Post\u2010mortem investigation into a death involving doping agents: The case of a body builder"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2176-6876","authenticated-orcid":false,"given":"Donata","family":"Favretto","sequence":"first","affiliation":[{"name":"Legal Medicine and Toxicology University Hospital of Padua  Padua Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giulia","family":"Stocchero","sequence":"additional","affiliation":[{"name":"Toxicology Sector Provincial Health Services Agency  Trento Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto","family":"Pertile","sequence":"additional","affiliation":[{"name":"Legal Medicine and Toxicology University Hospital of Padua  Padua Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raffaella","family":"Stimamiglio","sequence":"additional","affiliation":[{"name":"Legal Medicine and Toxicology University Hospital of Padua  Padua Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonello","family":"Cirnelli","sequence":"additional","affiliation":[{"name":"Specialist in Legal Medicine in Portogruaro  Portogruaro Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7870-7362","authenticated-orcid":false,"given":"Maddalena","family":"Galeazzi","sequence":"additional","affiliation":[{"name":"School of Specialisation in Legal Medicine University Hospital of Padua  Padua Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,8,14]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1249\/00005768\u2010199508000\u201000003"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/bkz058"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jemermed.2012.02.057"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.2174\/1570159X13666141210222725"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/sms.13221"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/bky088"},{"key":"e_1_2_8_8_1","unstructured":"National Institute on Drug Abuse website (NIDA).2021What are the side effects of anabolic steroid misuse?. 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