{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T03:26:20Z","timestamp":1764645980668,"version":"build-2065373602"},"reference-count":19,"publisher":"Wiley","issue":"3-4","license":[{"start":{"date-parts":[[2011,10,13]],"date-time":"2011-10-13T00:00:00Z","timestamp":1318464000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:p>Atropine is used in the daily clinical practice for the treatment of poisonings caused by anticholinesterase pesticides, due to its sympathomimetic action. The investigation of the cause of the adverse effects that appear during atropine administration showed the necessity for the development and validation of a simple, rapid, sensitive, and specific method for the determination of atropine levels in serum samples. The developed method includes liquid\u2010liquid extraction with ethyl acetate: dichloromethane (3:1, <jats:italic>v\/v<\/jats:italic>) and derivatization using N,O\u2010bis(trimethylsilyl)\u2010trifluoracetamide (BSTFA) with 1% trimethylchlorsilane (TMCS) in acetonitrile environment. The method was found to be selective, linear, accurate, and precise according to international guidelines. The recovery was higher than 85.9%, the limit of quantification was 2.00\u2009ng\/ml, and the calibration curve was linear within the range of 2.00\u2013500\u2009ng\/ml (R<jats:sup>2<\/jats:sup>\u2009\u2265\u20090.992). Accuracy and precision were also calculated and were found to be less than 5.2 and 8.7%, respectively. The developed method was applied in a real case of accidental poisoning with chlorpyrifos in order to determine the atropine serum levels of the patient. The proposed method proved to be useful for the investigation of adverse effects that appear during atropine treatment of patients poisoned by anticholinesterase pesticides and it can also be used for the investigation of poisonings caused after consumption of atropine containing plants. Copyright \u00a9 2011 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/dta.343","type":"journal-article","created":{"date-parts":[[2011,10,13]],"date-time":"2011-10-13T09:19:07Z","timestamp":1318497547000},"page":"229-234","source":"Crossref","is-referenced-by-count":15,"title":["A simple and sensitive GC\/MS method for the determination of atropine during therapy of anticholinesterase poisoning in serum samples"],"prefix":"10.1002","volume":"4","author":[{"given":"Ioannis","family":"Papoutsis","sequence":"first","affiliation":[{"name":"Department of Forensic Medicine and Toxicology School of Medicine National and Kapodistrian University of Athens  Athens 11527 Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Panagiota","family":"Nikolaou","sequence":"additional","affiliation":[{"name":"Department of Forensic Medicine and Toxicology School of Medicine National and Kapodistrian University of Athens  Athens 11527 Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chara","family":"Spiliopoulou","sequence":"additional","affiliation":[{"name":"Department of Forensic Medicine and Toxicology School of Medicine National and Kapodistrian University of Athens  Athens 11527 Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Constantinos","family":"Pistos","sequence":"additional","affiliation":[{"name":"Department of Forensic Medicine and Toxicology School of Medicine National and Kapodistrian University of Athens  Athens 11527 Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Stefanidou","sequence":"additional","affiliation":[{"name":"Department of Forensic Medicine and Toxicology School of Medicine National and Kapodistrian University of Athens  Athens 11527 Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sotirios","family":"Athanaselis","sequence":"additional","affiliation":[{"name":"Department of Forensic Medicine and Toxicology School of Medicine National and Kapodistrian University of Athens  Athens 11527 Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,10,13]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2009.07.025"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007691-200202000-00022"},{"key":"e_1_2_5_4_1","first-page":"642","article-title":"Pharmacological agents in the prophylaxis\/treatment of organophosphorous pesticide intoxication","volume":"48","author":"Husain K.","year":"2010","journal-title":"Indian J. 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