{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T11:40:07Z","timestamp":1768477207265,"version":"3.49.0"},"reference-count":33,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,3,22]],"date-time":"2023-03-22T00:00:00Z","timestamp":1679443200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The combination of sulfadoxine (SDO) with trimethoprim (TMP) is widely used in veterinarian medicine. The aim of the present study was to compare excretion profiles and detection time windows of SDO and TMP in plasma and urine by means of a validated quantitative method.<\/jats:p><jats:p>Eight horses received a single intravenous (i.v.) dose of 2.7\u2009mg TMP and 13.4\u2009mg SDO per kg bodyweight. Plasma and urine samples were collected up to 15 and 70 days post\u2010administration, respectively.<\/jats:p><jats:p>While urine samples underwent an enzymatic hydrolysis, plasma samples were proteolysed before further analysis. After solid\u2010phase extraction, samples were analysed by liquid chromatography\/electrospray ionisation tandem mass spectrometry in positive ionisation mode. The applied multiple reaction monitoring (MRM) method allowed the detection of SDO and TMP with a lower limit of detection of 0.03\u2009ng\/mL in plasma and 0.2 (SDO) and 0.4\u2009ng\/mL (TMP) in urine, respectively. In the present study, detection times for SDO were 15\u2009days in plasma and 49\u2009days in urine, respectively. TMP was detected for up to 7\u2009days in plasma and up to 50\u2009days in urine, respectively. The detection via the TMP metabolite 3\u2010desmethyl\u2010trimethoprim was possible for 70\u2009days in urine. Detection times of the other confirmed metabolites N<jats:sub>4<\/jats:sub>\u2010acetylated sulfadoxine, hydroxytrimethoprim, trimethoprim\u20101\u2010oxide and trimethoprim\u20103\u2010oxide were significantly lower.<\/jats:p><jats:p>In order to postulate reasonable screening limits (SLs) to control specific withdrawal times, a Monte Carlo simulation was performed for SDO. The proposed SL of 10\u2009ng\/mL SDO in blood and 300\u2009ng\/mL urine corresponds to a detection time of 4\u2009days.<\/jats:p>","DOI":"10.1002\/dta.3461","type":"journal-article","created":{"date-parts":[[2023,2,20]],"date-time":"2023-02-20T23:46:05Z","timestamp":1676936765000},"page":"629-645","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Control of a sulfadoxine\/trimethoprim combination in the competition horse: Elimination, metabolism and detection following an intravenous administration"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7485-3466","authenticated-orcid":false,"given":"Ina","family":"Schenk","sequence":"first","affiliation":[{"name":"Institute of Biochemistry, Center for Preventive Doping Research German Sport University Cologne  Cologne 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