{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:39:58Z","timestamp":1786977598029,"version":"build-2736575974"},"reference-count":38,"publisher":"Wiley","issue":"11-12","license":[{"start":{"date-parts":[[2023,3,14]],"date-time":"2023-03-14T00:00:00Z","timestamp":1678752000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100013935","name":"Partnership for Clean Competition","doi-asserted-by":"publisher","award":["2019R2000409G"],"award-info":[{"award-number":["2019R2000409G"]}],"id":[{"id":"10.13039\/100013935","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Sulfated metabolites have shown to have potential as long\u2010term markers (LTMs) of anabolic\u2013androgenic steroid (AAS) abuse. The compatibility of gas chromatography\u2013mass spectrometry (GC\u2013MS) with trimethylsilyl (TMS)\u2010derivatives of non\u2010hydrolysed sulfated steroids has been demonstrated, where, after derivatisation, generally, two closely eluting isomers are formed that both have the same molecular ion [M\u2010H\n                    <jats:sub>2<\/jats:sub>\n                    SO\n                    <jats:sub>4<\/jats:sub>\n                    ]\n                    <jats:sup>\u2022+<\/jats:sup>\n                    . Sulfated reference standards are in limited commercial availability, and therefore, the current knowledge of the GC\u2013MS behaviour of these compounds is mainly based on sulfating and analysing the available standard reference material. This procedure can unfortunately not cover all of the current known LTMs as these are often not available as pure substance. Therefore, in theory, some metabolites could be missed as they exhibit alternative behaviour. To investigate the matter, in\u2010house sulfated reference materials that bear resemblance to known sulfated LTMs were analysed on GC\u2013MS in their TMS\u2010derivatised non\u2010hydrolysed state. The (alternative) gas chromatographic and mass spectrometric behaviour was mapped, evaluated and linked to the corresponding steroid structures. Afterwards, using fraction collection, known sulfated LTMs were isolated from excretion urine to confirm the observed findings. The categories that were selected were mono\u2010hydroxy\u2010diones, 17\u2010methyl\u20103,17\u2010diols and 17\u2010keto\u20103,16\u2010diols as these are commonly encountered AAS conformations. The ability to predict the GC\u2013MS behaviour of non\u2010hydrolysed sulfated AAS metabolites is the corner stone of finding new metabolites. This knowledge is also essential, for example, for understanding AAS detection analyses, for the mass spectrometric characterization of metabolites of new designer steroids or when one needs to characterize an unknown steroid structure.\n                  <\/jats:p>","DOI":"10.1002\/dta.3462","type":"journal-article","created":{"date-parts":[[2023,2,27]],"date-time":"2023-02-27T02:34:15Z","timestamp":1677465255000},"page":"1344-1355","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Evaluation of alternative gas chromatographic and mass spectrometric behaviour of trimethylsilyl\u2010derivatives of non\u2010hydrolysed sulfated anabolic steroids"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2748-6197","authenticated-orcid":false,"given":"A\u00f0alhei\u00f0ur D\u00f3ra","family":"Albertsd\u00f3ttir","sequence":"first","affiliation":[{"name":"Doping Control Laboratory (DoCoLab), 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