{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T15:08:17Z","timestamp":1791385697866,"version":"4.3.3"},"reference-count":46,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,5,10]],"date-time":"2023-05-10T00:00:00Z","timestamp":1683676800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005416","name":"Norges Forskningsr\u00e5d","doi-asserted-by":"publisher","award":["NRF 310086"],"award-info":[{"award-number":["NRF 310086"]}],"id":[{"id":"10.13039\/501100005416","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Separation and quantification of amphetamine enantiomers are commonly used to distinguish between consumption of prescription amphetamine (mostly\n                    <jats:italic>S<\/jats:italic>\n                    \u2010amphetamine) and illicit forms of the drug (racemate). In this study, electromembrane extraction with prototype conductive vials was combined with ultra\u2010high performance supercritical fluid chromatography (UHPSFC\u2010MS\/MS) to quantify\n                    <jats:italic>R<\/jats:italic>\n                    \u2010 and\n                    <jats:italic>S<\/jats:italic>\n                    \u2010amphetamine in urine. Amphetamine was extracted from 100\u2009\u03bcL urine, diluted with 25\u2009\u03bcL internal standard solution and 175\u2009\u03bcL 130\u2009mM formic acid, across a supported liquid membrane (SLM) consisting of 9\u00a0\u03bcL of a 1:1(w\/w) mixture of 2\u2010nitrophenyloctyl ether (NPOE) and bis(2\u2010ethylhexyl)phosphite (DEHPi) into an acceptor phase containing 300\u2009\u03bcL 130\u2009mM formic acid. The extraction was facilitated by the application of 30\u2009V for 15\u2009min. Enantiomeric separation was achieved using UHPSFC\u2010MS\/MS with a chiral stationary phase. The calibration range was 50\u201310,000\u2009ng\/mL for each enantiomer. The between\u2010assay CV was \u22645%, within\u2010assay CV\u2009\u2264\u20091.5%, and bias within \u00b12%. Recoveries were 83%\u201390% (CV\u2009\u2264\u20096%), and internal standard corrected matrix effects were 99\u2013105 (CV\u2009\u2264\u20092%). The matrix effects ranged from 96% to 98% (CV\u2009\u2264\u20098%) when not corrected by the internal standard. The EME method was compared with a chiral routine method that employed liquid\u2013liquid extraction (LLE) for sample preparation. Assay results were in agreement with the routine method, and the mean deviation between methods was 3%, ranging from \u221221% to 31%. Finally, sample preparation greenness was assessed using the AGREEprep tool, which resulted in a greenness score of 0.54 for conductive vial EME, opposed to 0.47 for semi\u2010automated 96\u2010well LLE.\n                  <\/jats:p>","DOI":"10.1002\/dta.3487","type":"journal-article","created":{"date-parts":[[2023,4,28]],"date-time":"2023-04-28T05:59:07Z","timestamp":1682661547000},"page":"909-918","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Determination of amphetamine enantiomers in urine by conductive vial electromembrane extraction and ultra\u2010high performance supercritical fluid chromatography tandem mass spectrometry"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9146-2870","authenticated-orcid":false,"given":"Tonje Gottenberg","family":"Skaalvik","sequence":"first","affiliation":[{"name":"Department of Clinical Pharmacology St. Olav University Hospital  Trondheim Norway"},{"name":"Department of Pharmacy University of Oslo  Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0781-6865","authenticated-orcid":false,"given":"Elisabeth Leere","family":"\u00d8iestad","sequence":"additional","affiliation":[{"name":"Department of Pharmacy University of Oslo  Oslo Norway"},{"name":"Department of Forensic Sciences, Division of Laboratory Medicine Oslo University Hospital  Oslo Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stig","family":"Pedersen\u2010Bjergaard","sequence":"additional","affiliation":[{"name":"Department of Pharmacy University of Oslo  Oslo Norway"},{"name":"Department of Pharmacy, Faculty of Health and Medical Sciences University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6334-6357","authenticated-orcid":false,"given":"Solfrid","family":"Hegstad","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology St. Olav University Hospital  Trondheim Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,5,10]]},"reference":[{"key":"e_1_2_7_2_1","unstructured":"The Norwegian Prescription Data Base.2023.https:\/\/www.reseptregisteret.no\/Prevalens.aspx"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3118"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jksus.2020.05.003"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/24.3.223"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.2879"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2018.01.028"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2018.08.061"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2013.12.014"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talo.2022.100171"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.7b01284"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2014.04.038"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.8b02292"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2017.10.049"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10404\u2010010\u20100603\u20106"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2021.122926"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sampre.2022.100011"},{"key":"e_1_2_7_18_1","unstructured":"U.S Department of Health and Human Services.Food and Drug Administration. 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