{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T01:06:55Z","timestamp":1760404015167,"version":"build-2065373602"},"reference-count":46,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,7,4]],"date-time":"2023-07-04T00:00:00Z","timestamp":1688428800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Phosphatidylethanol (PEth) is a non\u2010oxidative metabolite of alcohol (ethanol), which is a sensitive and specific indicator of historic ethanol consumption. Although PEth production from ethanol is catalysed by the ubiquitous enzyme phospholipase D, it resides mainly within the erythrocyte compartment of the blood. PEth analysis has been reported in different preparations of whole blood, representing one of the barriers of inter\u2010laboratory comparisons. We previously reported that expressing PEth concentrations in terms of blood erythrocyte content is more sensitive than whole blood volume, and haematocrit\u2010corrected liquid whole blood calculations of erythrocyte PEth and isolated erythrocyte PEth concentrations are comparable when assayed under identical analytical conditions. Acceptance of a clinical diagnostic assay by accreditation bodies requires proficiency testing with a third\u2010party analytical facility. To explore different blood preparations within the same inter\u2010laboratory program, 60 matched isolated erythrocyte or liquid whole blood specimens were tested at three laboratories. Laboratories measured PEth by liquid chromatography\u2010tandem mass spectrometry (LC\u2010MS\/MS), two using isolated erythrocytes, while the third used liquid whole blood, which underwent haematocrit correction before comparison with isolated erythrocyte PEth concentrations. There was acceptable consensus (87%) among laboratories to detect PEth around a cut\u2010off of 35\u2009\u03bcg\/L of erythrocytes. Each laboratory correlated well with the group average PEth concentration (R\u2009&gt;\u20090.98) for each specimen above the cut\u2010off. Differences were observed between laboratories in bias, which did not affect comparable sensitivity at the selected cut\u2010off. This work demonstrates the feasibility of an inter\u2010laboratory comparison for erythrocyte PEth analysis across different LC\u2010MS\/MS methodologies and different blood preparations.<\/jats:p>","DOI":"10.1002\/dta.3537","type":"journal-article","created":{"date-parts":[[2023,7,4]],"date-time":"2023-07-04T21:53:18Z","timestamp":1688507598000},"page":"251-258","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["LC\u2010MS\/MS analysis of erythrocyte phosphatidylethanol in haematocrit\u2010corrected whole blood versus isolated erythrocytes: Results of an inter\u2010laboratory comparison"],"prefix":"10.1002","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9487-239X","authenticated-orcid":false,"given":"Daniel","family":"White","sequence":"first","affiliation":[{"name":"Department of Clinical Pharmacology and Toxicology PathWest Laboratory Medicine  Nedlands Western Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5797-6854","authenticated-orcid":false,"given":"Michael","family":"Fitzpatrick","sequence":"additional","affiliation":[{"name":"Chemical Pathology, NSW Health Pathology Royal Prince Alfred Hospital  Camperdown New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brett","family":"McWhinney","sequence":"additional","affiliation":[{"name":"Chemical Pathology, Pathology Queensland Royal Brisbane and Women's Hospital  Herston Queensland Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sam","family":"Salman","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology and Toxicology PathWest Laboratory Medicine  Nedlands Western Australia"},{"name":"School of Medicine and School of Biomedical Sciences University of Western Australia  Crawley Western Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David A.","family":"Joyce","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology and Toxicology PathWest Laboratory Medicine  Nedlands Western Australia"},{"name":"School of Medicine and School of Biomedical Sciences University of Western Australia  Crawley Western Australia Australia"},{"name":"Sir Charles Gairdner Hospital  Nedlands Western Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,7,4]]},"reference":[{"volume-title":"International guide for monitoring alcohol consumption and related harm","year":"2000","author":"World Health 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