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To start addressing this need, a method measuring the inhibition of the dopamine, serotonin, and norepinephrine transporters (DAT, SERT, and NET) by stimulant drugs was developed. The use of a proprietary fluorescent dye mixture and three cell lines (CHO\u2010K1, HEK 293, and MDCK), each expressing a single transporter, allowed for a semiautomated, one\u2010pot determination of inhibition in a 384\u2010well format. The method was validated using well characterized stimulants, including cocaine, amphetamine, 3,4\u2010methylenedioxymethamphetamine (MDMA), \u03b1\u2010PVP, and fluoxetine and performed similarly to other methods. Seven synthetic cathinones all showed highest potency for DAT inhibition, followed by NET and SERT. The rank potency for DAT inhibition IC\n                    <jats:sub>50<\/jats:sub>\n                    (nM) was MPHP (4.53)\u2009&gt;\u20094Cl\u2010\u03b1\u2010PVP (8.05)\u2009&gt;\u20093F\u2010\u03b1\u2010PVP (12.7)\u2009&gt;\u2009\u03b1\u2010PiHP (13.4)\u2009&gt;\u2009\n                    <jats:italic>N<\/jats:italic>\n                    \u2010ethylpentylone (16.9)\u2009&gt;\u2009\n                    <jats:italic>N<\/jats:italic>\n                    \u2010ethylhexedrone (44.5)\u2009&gt;\u20094\u2010methylpentedrone (261). All but 4\u2010methylpentedrone were more potent than amphetamine (257) and cocaine (111). The DAT\/SERT inhibition ratio for the cathinones was in the range from 5.02 for 4\u2010methylpentedrone to &gt;3730 for \u03b1\u2010PiHP, compared to 1.64 for cocaine and &gt;4030 for \u03b1\u2010PVP. All seven substances had inhibition profiles similar to those of potent stimulants with high abuse potential.\n                  <\/jats:p>","DOI":"10.1002\/dta.3547","type":"journal-article","created":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T02:50:51Z","timestamp":1690253451000},"page":"339-347","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Characterization of neurotransmitter inhibition for seven cathinones by a proprietary fluorescent dye method"],"prefix":"10.1002","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4383-2902","authenticated-orcid":false,"given":"Mattias","family":"Persson","sequence":"first","affiliation":[{"name":"Department of Forensic Genetics and Forensic Toxicology National Board of Forensic Medicine  Link\u00f6ping Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5977-3049","authenticated-orcid":false,"given":"Svante","family":"Vikingsson","sequence":"additional","affiliation":[{"name":"Department of Forensic Genetics and Forensic Toxicology National Board of Forensic Medicine  Link\u00f6ping Sweden"},{"name":"Division of Clinical Chemistry and Pharmacology, Department of Biomedical and Clinical Sciences, Faculty of Medicine and Health Sciences Link\u00f6ping University  Link\u00f6ping Sweden"},{"name":"Center for Forensic Science Advancement and Application, RTI International  Research Triangle Park North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4222-9597","authenticated-orcid":false,"given":"Robert","family":"Kronstrand","sequence":"additional","affiliation":[{"name":"Department of Forensic Genetics and Forensic Toxicology National Board of Forensic Medicine  Link\u00f6ping Sweden"},{"name":"Division of Clinical Chemistry and Pharmacology, Department of Biomedical and Clinical Sciences, Faculty of Medicine and Health Sciences Link\u00f6ping University  Link\u00f6ping Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8015-5728","authenticated-orcid":false,"given":"Henrik","family":"Green","sequence":"additional","affiliation":[{"name":"Department of Forensic Genetics and Forensic Toxicology National Board of Forensic Medicine  Link\u00f6ping Sweden"},{"name":"Division of Clinical Chemistry and Pharmacology, Department of Biomedical and Clinical Sciences, Faculty of Medicine and Health Sciences Link\u00f6ping University  Link\u00f6ping Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,7,24]]},"reference":[{"key":"e_1_2_7_2_1","unstructured":"European Monitoring Centre for Drugs and Drug Addiction (2022).European drug report 2022: trends and developments. 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