{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T17:11:30Z","timestamp":1790183490961,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2023,12,3]],"date-time":"2023-12-03T00:00:00Z","timestamp":1701561600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Phosphatidylethanol (PEth) has recently become a popular direct alcohol marker for evaluating drinking behavior. This study aimed at gaining further information on the long\u2010term stability of five PEth homologues (16:0\/18:1, 16:0\/18:2, 16:0\/20:4, 18:0\/18:1, 18:0\/18:2) in whole blood (WB) and dried blood spots (DBS) stored at \u221280\u00b0C, 4\u00b0C, and room temperature (18\u00b0C) over a period of 60\u2009days. Venous blood was taken from 10 volunteers (five females and five males, aged 21\u201340\u2009years) with a moderate drinking behavior and a negative breath alcohol test at the time of collection. 100 \u03bcL aliquots of WB were prepared in addition to 20 \u03bcL DBS samples. The initial PEth concentrations were determined on the day of the blood collection. On days 1, 3, 5, 7, 11, 17, 40, and 60, DBS were analyzed in triplicate by means of LC\u2013MS\/MS. On these days, WB aliquots having been stored until that time were used to create further DBS in triplicate, which were subsequently stored at 18\u00b0C and analyzed in a single batch after day 60. All homologues, except PEth 16:0\/20:4, were stable at \u221280\u00b0C in DBS and WB for 60\u2009days. The initial PEth 16:0\/18:1 concentrations remained stable in both DBS and WB in all but one volunteer's specimen at 4 and 18\u00b0C. Apart from this exception, simultaneously detected PEth homologues 16:0\/18:2, 18:0\/18:1, and 18:0\/18:2 remained stable over at least 40\u2009days in DBS. Nevertheless, the storage time between sample collection and analysis should be kept as short as possible if an ethanol\u2010free sample cannot be ensured.<\/jats:p>","DOI":"10.1002\/dta.3619","type":"journal-article","created":{"date-parts":[[2023,12,3]],"date-time":"2023-12-03T21:11:52Z","timestamp":1701637912000},"page":"968-976","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Storage stability of phosphatidylethanol homologues in whole blood and dried blood spots of nonalcoholics at different temperatures over 60\u2009days"],"prefix":"10.1002","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4955-2888","authenticated-orcid":false,"given":"Josefine","family":"Herzog","sequence":"first","affiliation":[{"name":"Forensic Toxicological Center (FTC) Munich  Munich 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