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Briefly, sample preparation involved an alkaline liquid\u2013liquid extraction (methyl tert\u2010butyl ether) of blood (100\u2009\u03bcl). The solvent layer was transferred, evaporated to dryness, reconstituted, and samples then separated on an Agilent Poroshell 120 EC\u2010C18 100\u2009\u00c5 (50\u2009mm\u2009\u00d7\u20093.0\u00a0mm, 2.7\u00a0\u03bcm) analytical column using a multi\u2010step gradient elution of 50\u2009mM ammonium formate in water (pH\u00a03.5) and 0.1% formic acid in methanol over 14\u2009min. A SCIEX Triple Quad 6500+\u2009system operating in scheduled multiple reaction monitoring and positive electrospray ionization was used for detection. There were no interferences, and matrix effects were generally acceptable (\u00b120% of neat response). Linearity was achieved within the calibration range, including methylamphetamine (MA) (10\u20131000\u2009ng\/ml), 3,4\u2010methylenedioxy\u2010N\u2010methylamphetamine (MDMA) (10\u20131,000\u2009ng\/ml), cocaine (10\u20131000\u2009ng\/ml), and two THC isomers (1\u2013100\u2009ng\/ml). Accuracies of MA, MDMA, cocaine, and two THC isomers were 3.6 to 8.9%, \u22121.2 to 4%, \u22125.3 to 5.8%, and \u221211 to 14%, respectively; while precision estimates of the same were 1.6 to 5.4%, 1.7 to 5.3%, 1.2 to 4.5%, and 2 to 10%, respectively. Autosampler\u00a0stability and dilution integrity were within acceptable limits, and no carryover was detected at the limit of detection. This validated LC\u2010MS\/MS method made the routine identification of both delta\u20109\u2010THC and delta\u20108\u2010THC in blood possible.<\/jats:p>","DOI":"10.1002\/dta.3632","type":"journal-article","created":{"date-parts":[[2023,12,30]],"date-time":"2023-12-30T05:53:34Z","timestamp":1703915614000},"page":"1102-1112","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Quantitative analysis of tetrahydrocannabinol isomers and other toxicologically relevant drugs in blood"],"prefix":"10.1002","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8943-8994","authenticated-orcid":false,"given":"Dylan","family":"Mantinieks","sequence":"first","affiliation":[{"name":"Department of Forensic Medicine School of Public Health and Preventive Medicine Monash University  Southbank Victoria Australia"},{"name":"Victorian Institute of Forensic Medicine  Southbank Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3490-5214","authenticated-orcid":false,"given":"Matthew","family":"Di Rago","sequence":"additional","affiliation":[{"name":"Department of Forensic Medicine School of Public Health and Preventive Medicine Monash University  Southbank Victoria Australia"},{"name":"Victorian Institute of Forensic Medicine  Southbank Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8953-0312","authenticated-orcid":false,"given":"Olaf H.","family":"Drummer","sequence":"additional","affiliation":[{"name":"Department of Forensic Medicine School of Public Health and Preventive Medicine Monash University  Southbank Victoria Australia"},{"name":"Victorian Institute of Forensic Medicine  Southbank Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7161-7513","authenticated-orcid":false,"given":"Linda","family":"Glowacki","sequence":"additional","affiliation":[{"name":"Victorian Institute of Forensic Medicine  Southbank Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8870-6272","authenticated-orcid":false,"given":"Jennifer","family":"Schumann","sequence":"additional","affiliation":[{"name":"Department of Forensic Medicine School of Public Health and Preventive Medicine Monash University  Southbank Victoria Australia"},{"name":"Victorian Institute of Forensic Medicine  Southbank Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7958-8235","authenticated-orcid":false,"given":"Dimitri","family":"Gerostamoulos","sequence":"additional","affiliation":[{"name":"Department of Forensic Medicine School of Public Health and Preventive Medicine Monash University  Southbank Victoria Australia"},{"name":"Victorian Institute of Forensic Medicine  Southbank Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,12,30]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/bkab029"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja00960a056"},{"issue":"31","key":"e_1_2_8_4_1","article-title":"Delta\u20108\u2010THC craze concerns chemists","volume":"99","author":"Erickson BE","year":"2021","journal-title":"Chem Eng News"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27206924"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/cpt1973143353"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1159\/000136375"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.15865"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s42238\u2010023\u201000191\u2010y"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s42238\u2010021\u201000115\u20108"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2022.114987"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2019.03.002"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/bkac007"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2022.113899"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aap.2012.06.017"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/bkaa057"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.forsciint.2014.03.021"},{"key":"e_1_2_8_18_1","volume-title":"American Academy of forensic sciences standards board. 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