{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T16:50:46Z","timestamp":1790095846798,"version":"4.0.1"},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,3,23]],"date-time":"2024-03-23T00:00:00Z","timestamp":1711152000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Testosterone, nandrolone, and boldenone, which are listed as doping substances on the World Anti\u2010Doping Agency Prohibited List, are mostly available commercially in esterified forms. Isotope ratio mass spectrometry (IRMS) represents a key tool for identifying these substances, as they are hydrolyzed and discharged in the urine as pseudo\u2010endogenous substances. However, IRMS, which comprises a complicated process, cannot achieve the direct detection of steroid esters in blood samples. These substances can be detected using dried blood spots (DBSs), reducing the impact of esterase hydrolysis. Here, a simultaneous liquid chromatography\u2013tandem mass spectrometry method for detecting 28 steroid (13 testosterone, nine nandrolone, and six boldenone) esters was developed using three DBS types of samples, including a cellulose paper and polymer. The substances were first derivatized with methyloxime to increase their sensitivities (the limits of detection were &lt;0.1\u20130.4, &lt;0.1\u20130.9, and &lt;0.1\u20130.9\u00a0ng\/mL for the testosterone, nandrolone, and boldenone esters, respectively). Further, the DBS absorbents were verified since the effect of interferences depended on it. Next, a study involving seven participants was conducted to detect intramuscularly administered testosterone enanthate (100\u2009mg). Polymer and cellulose papers were used to collect blood from their upper arms and fingertips, respectively, and testosterone enanthate was identified and detectable at both blood\u2010collection sites for up to 144 and 216\u2009h, respectively. Furthermore, testosterone enanthate was detectable in the DBS samples stored under refrigeration after 6\u2009months, indicating the stable nature of DBS.<\/jats:p>","DOI":"10.1002\/dta.3681","type":"journal-article","created":{"date-parts":[[2024,3,23]],"date-time":"2024-03-23T05:18:26Z","timestamp":1711171106000},"page":"42-55","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Simultaneous detection of testosterone, nandrolone, and boldenone esters in dried blood spots for doping control in sports by liquid chromatography\u2013tandem mass spectrometry"],"prefix":"10.1002","volume":"17","author":[{"given":"Asami","family":"Miyamoto","sequence":"first","affiliation":[{"name":"Anti\u2010Doping Laboratory LSI Medience Corporation  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masanori","family":"Ota","sequence":"additional","affiliation":[{"name":"Anti\u2010Doping Laboratory LSI Medience Corporation  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitsuhiko","family":"Sato","sequence":"additional","affiliation":[{"name":"Anti\u2010Doping Laboratory LSI Medience Corporation  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3614-4121","authenticated-orcid":false,"given":"Masato","family":"Okano","sequence":"additional","affiliation":[{"name":"Anti\u2010Doping Laboratory LSI Medience Corporation  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,3,23]]},"reference":[{"key":"e_1_2_5_2_1","unstructured":"World Anti\u2010Doping Agency.World Anti\u2010Doping Code. International Standard. Prohibited List 2023.2021. Accessed December 4 2023.https:\/\/www.wada-ama.org\/sites\/default\/files\/2023-05\/2023list_en_final_9_september_2022.pdf"},{"key":"e_1_2_5_3_1","unstructured":"World Anti\u2010Doping Agency.Measurement and reporting of endogenous anabolic androgenic steroid (EAAS) markers of the urinary steroid profile. WADA Technical Document\u2014TD2021EAAS.2021. Accessed December 4 2023.https:\/\/www.wada-ama.org\/sites\/default\/files\/2022-01\/td2021eaas_final_eng_v_2.0.pdf"},{"key":"e_1_2_5_4_1","unstructured":"World Anti\u2010Doping Agency.Detection of Synthetic Forms of Prohibited Substances by GC\/C\/IRMS. WADA Technical Document\u2014TD2022IRMS.2022. Accessed December 4 2023.https:\/\/www.wada\u2010ama.org\/sites\/default\/files\/2022\u201001\/td2022irms_v1.0_final_eng_0_0.pdf"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.1560"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.1914"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2020.113807"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3379"},{"key":"e_1_2_5_9_1","unstructured":"World Anti\u2010Doping Agency.Dried blood spots (DBS) for doping control. WADA Technical Document\u2014TD2023DBS.2023. Accessed December 4 2023.https:\/\/www.wada-ama.org\/sites\/default\/files\/2022-11\/td2023dbs_cleanversion.pdf"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2014.03.013"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3030"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2022.123535"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3226"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.5633"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3384"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3135"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102125.2010.03668.x"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.2337\/diacare.25.2.337"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3414"},{"key":"e_1_2_5_20_1","unstructured":"World Anti\u2010Doping Agency.Minimum criteria for chromatographic\u2010mass spectrometric confirmation of the identify if analytes for doping control purposes. WADA Technical Document\u2014TD2023IDCR.2023. Accessed December 4 2023.https:\/\/www.wada-ama.org\/sites\/default\/files\/2023-02\/td2023idcrv1.1_eng_final.pdf"},{"key":"e_1_2_5_21_1","unstructured":"World Anti\u2010Doping Agency.The World Anti\u2010Doping Code. International Standard for Laboratories (ISL) 2021.2021. Accessed December 4 2023.https:\/\/www.wada-ama.org\/sites\/default\/files\/resources\/files\/isl_2021.pdf"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3367"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba"},{"key":"e_1_2_5_24_1","unstructured":"MiyamotoA ShiomuraS OkanoM DohiM KageyamaS.Detection of testosterone esters in dried blood spot (DBS) on sports doping testing. Poster presented at: The 41st Annual Meeting of the Japanese Society for Biomedical Mass Spectrometry; September 15 2016; Aichi Japan."},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2014\u20101243"}],"container-title":["Drug Testing and Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dta.3681","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T20:29:52Z","timestamp":1760387392000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/dta.3681"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3,23]]},"references-count":24,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1002\/dta.3681"],"URL":"https:\/\/doi.org\/10.1002\/dta.3681","archive":["Portico"],"relation":{},"ISSN":["1942-7603","1942-7611"],"issn-type":[{"value":"1942-7603","type":"print"},{"value":"1942-7611","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3,23]]},"assertion":[{"value":"2023-12-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}