{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:05:32Z","timestamp":1771459532878,"version":"3.50.1"},"reference-count":23,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,4,11]],"date-time":"2024-04-11T00:00:00Z","timestamp":1712793600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Doping analyses are essential for sporting events because some athletes might use prohibited substances to win games. To obtain reliable results from doping analyses, it is important to use both reliable standard solutions and validated analytical methods at accredited laboratories. Among the focused compounds related to prohibited substances listed by the World Anti\u2010Doping Agency, we developed a certified reference material (CRM) for 3<jats:italic>\u03b2<\/jats:italic>,4<jats:italic>\u03b1<\/jats:italic>\u2010dihydroxy\u20105<jats:italic>\u03b1<\/jats:italic>\u2010androstan\u201017\u2010one (DHAS), a metabolite of formestane that is used to conceal prohibited anabolic steroids, in methanol solution (NMIJ CRM 6212\u2010a). To develop a CRM traceable to the International System of Units (SI), we newly applied different analytical methods with an SI\u2010traceable internal standard for quantitative NMR (qNMR) instead of mass balance approach because this CRM solution was required to develop rapidly using a limited amount of high\u2010purity DHAS. One method was gravimetric blending using the purity of DHAS powder evaluated by both qNMR and a combination of qNMR and high\u2010performance liquid chromatography (HPLC), and the other was direct quantification of the DHAS mass fraction in the candidate solution CRM by both qNMR and qNMR\/HPLC. Because the values obtained by gravimetric blending and direct quantification of the mass fraction were comparable, the arithmetic mean was applied to obtain the certified value. Considering homogeneity and stability according to ISO Guide 35: 2017, the certified values with expanded uncertainties (coverage factor <jats:italic>k<\/jats:italic>\u2009=\u20092, approximate 95% confidence interval) were (135.2\u2009\u00b1\u20099.5) \u03bcg\/g for the mass fraction and (107.0\u2009\u00b1\u20097.5) \u03bcg\/ml for the mass concentration.<\/jats:p>","DOI":"10.1002\/dta.3686","type":"journal-article","created":{"date-parts":[[2024,4,12]],"date-time":"2024-04-12T02:05:06Z","timestamp":1712887506000},"page":"177-187","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Development of 3<i>\u03b2<\/i>,4<i>\u03b1<\/i>\u2010dihydroxy\u20105<i>\u03b1<\/i>\u2010androstan\u201017\u2010one standard solution for doping analyses"],"prefix":"10.1002","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8827-5647","authenticated-orcid":false,"given":"Miho","family":"Kuroe","sequence":"first","affiliation":[{"name":"National Institute of Advanced Industrial Science and Technology (AIST) National Metrology Institute of Japan (NMIJ)  Tsukuba Ibaraki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7545-4467","authenticated-orcid":false,"given":"Taichi","family":"Yamazaki","sequence":"additional","affiliation":[{"name":"National Institute of Advanced Industrial Science and Technology (AIST) National Metrology Institute of Japan (NMIJ)  Tsukuba Ibaraki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8471-3451","authenticated-orcid":false,"given":"Naoki","family":"Saito","sequence":"additional","affiliation":[{"name":"National Institute of Advanced Industrial Science and Technology (AIST) National Metrology Institute of Japan (NMIJ)  Tsukuba Ibaraki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8564-3982","authenticated-orcid":false,"given":"Yoshitaka","family":"Shimizu","sequence":"additional","affiliation":[{"name":"National Institute of Advanced Industrial Science and Technology (AIST) National Metrology Institute of Japan (NMIJ)  Tsukuba Ibaraki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8864-8976","authenticated-orcid":false,"given":"Toshihide","family":"Ihara","sequence":"additional","affiliation":[{"name":"National Institute of Advanced Industrial Science and Technology (AIST) National Metrology Institute of Japan (NMIJ)  Tsukuba Ibaraki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4660-9682","authenticated-orcid":false,"given":"Masahiko","family":"Numata","sequence":"additional","affiliation":[{"name":"National Institute of Advanced Industrial Science and Technology (AIST) National Metrology Institute of Japan (NMIJ)  Tsukuba Ibaraki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0163-4454","authenticated-orcid":false,"given":"Nobuyasu","family":"Itoh","sequence":"additional","affiliation":[{"name":"National Institute of Advanced Industrial Science and Technology (AIST) National Metrology Institute of Japan (NMIJ)  Tsukuba Ibaraki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,4,11]]},"reference":[{"key":"e_1_2_7_2_1","unstructured":"World Anti\u2010Doping Code 2021. 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