{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T05:04:49Z","timestamp":1785128689246,"version":"3.55.0"},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,4,25]],"date-time":"2024-04-25T00:00:00Z","timestamp":1714003200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Phosphatidylethanol (PEth) measurement in whole blood samples is established as a specific alcohol biomarker with clinical and forensic applications. Establishment of dried blood spots (DBSs) as a specimen for PEth determination offers several advantages and was the focus of this work. A liquid chromatography tandem mass spectrometry method using a 96\u2010well format for sample preparation was developed and validated. PEth was extracted from DBSs by using isopropanol containing PEth\u2010d5 as internal standard. The blood sampling used a commercial volumetric DBS device having a phosholipase D inhibitor incorporated to stop continuous PEth formation. The method quantified PEth in the range of 0.05\u201310\u00a0\u03bcmol\/L, with a bias and imprecision of less than 15%. In a clinical study (<jats:italic>n<\/jats:italic>\u2009=\u200925) using fingerprick blood, the volumetric device offered more precise quantifications (CV 4.6%) compared with the Whatman 903 Protein Saver card device (CV 16.6%). In another clinical study (<jats:italic>n<\/jats:italic>\u2009=\u200948), the use of dried venous and capillary blood, and liquid venous blood was compared under real\u2010life conditions with samples sent by postal service. The capillary and venous DBS samples gave identical results while the liquid blood gave slightly higher values. Calculation of elimination half\u2010life (PEth 16:0\/18:1) in 31 cases based on two consecutive samples with 2\u20139\u00a0days in between gave results (mean 6.2\u00a0days) that agree with literature but several cases with values over 10\u00a0days. In conclusion, this study demonstrates that volumetric DBS is a valid specimen for determination of PEth blood concentrations, offering several advantages.<\/jats:p>","DOI":"10.1002\/dta.3695","type":"journal-article","created":{"date-parts":[[2024,4,25]],"date-time":"2024-04-25T20:33:30Z","timestamp":1714077210000},"page":"231-237","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Volumetric dried blood spots for determination of phosphatidylethanol: Validation of a liquid chromatography tandem masspectrometry method and clinical application"],"prefix":"10.1002","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3546-8872","authenticated-orcid":false,"given":"Olof","family":"Beck","sequence":"first","affiliation":[{"name":"Department of Clinical Neuroscience Karolinska Institutet  Stockholm Sweden"},{"name":"ABC Lab AB  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2518-3176","authenticated-orcid":false,"given":"Miguel","family":"Barroso","sequence":"additional","affiliation":[{"name":"ABC Lab AB  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sigurd","family":"Hermansson","sequence":"additional","affiliation":[{"name":"Waters AB  Solna Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristopher","family":"Wid\u00e9n","sequence":"additional","affiliation":[{"name":"ABC Lab AB  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christer","family":"Wallin","sequence":"additional","affiliation":[{"name":"Capitainer AB  Solna Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cecilia","family":"Nilsson\u2010Wallmark","sequence":"additional","affiliation":[{"name":"Department of Neuroscience and Physiology, Sect. Psychiatry and Neurochemistry, Sahlgrenska Academy, G\u00f6teborg University and Beroendekliniken Sahlgrenska University Hospital  Gothenburg Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"de Bejczy","sequence":"additional","affiliation":[{"name":"Department of Neuroscience and Physiology, Sect. Psychiatry and Neurochemistry, Sahlgrenska Academy, G\u00f6teborg University and Beroendekliniken Sahlgrenska University Hospital  Gothenburg Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,4,25]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.278"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms131114788"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/1556\u20104029.13874"},{"issue":"39","key":"e_1_2_8_5_1","first-page":"1747","article-title":"Nationell harmonisering av alkoholmark\u00f6ren PEth [National harmonization of the alcohol biomarker PEth]","volume":"110","author":"Helander A","year":"2013","journal-title":"Lakartidningen"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/alcalc\/agz064"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/acer.12883"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/acer.14362"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216\u2010011\u20105221\u2010y"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2018.01.008"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1039\/C0AY00636J"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/acer"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216\u2010015\u20109169\u20101"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.alc.0000145687.41646.e5"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00414\u2010012\u20100799\u2010y"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.alcohol.2018.03.003"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216\u2010021\u201003211\u2010z"},{"key":"e_1_2_8_18_1","volume-title":"User Verification of Precision and Estimation of Bias; Approved Guideline","author":"CLSI","year":"2014"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2017.11.036"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.8b03512"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1373\/jalm.2017.024828"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/alcalc\/agaa027"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1080\/00365513.2023.2253734"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/ejlt.201700362"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1177\/0004563220981106"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/jimd.12163"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41596\u2010020\u201000416\u2010x"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/alcalc\/ags065"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/alcalc\/agz027"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/acer.13062"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/bkaa034"}],"container-title":["Drug Testing and Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dta.3695","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T23:34:32Z","timestamp":1760398472000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/dta.3695"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,25]]},"references-count":30,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2025,2]]}},"alternative-id":["10.1002\/dta.3695"],"URL":"https:\/\/doi.org\/10.1002\/dta.3695","archive":["Portico"],"relation":{},"ISSN":["1942-7603","1942-7611"],"issn-type":[{"value":"1942-7603","type":"print"},{"value":"1942-7611","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,25]]},"assertion":[{"value":"2024-01-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}