{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T11:56:53Z","timestamp":1788782213119,"version":"build-2803163510"},"reference-count":27,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,7,8]],"date-time":"2025-07-08T00:00:00Z","timestamp":1751932800000},"content-version":"am","delay-in-days":365,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2024,7,8]],"date-time":"2024-07-08T00:00:00Z","timestamp":1720396800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","award":["LP180101000"],"award-info":[{"award-number":["LP180101000"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Previous liquid chromatography\/mass spectrometry (LC\/MS) methods for the detection of insulin and other similar peptide hormones in equine plasma relied on the use of antibody affinity extraction. As a result, these methods were not suitable for routine high\u2010throughput analysis. A solid\u2010phase extraction (SPE) method incorporating size exclusion as well as reversed\u2010phase interactions allows the selective extraction of peptide hormones such as adrenocorticotropic hormone (ACTH), insulin and their synthetic analogues from equine plasma with approximately 80% extraction efficiencies. This extraction was combined with on\u2010column derivatisation with acetic anhydride, followed by tryptic digestion and analysis by micro\u2010LC\/MSMS for high\u2010sensitivity peptide hormone detection. The analysis of tryptic peptides provides greater sensitivity and more robust chromatography compared with the analysis of intact insulin and ACTH. For quantitative analysis, isotopically labelled internal standards of target peptides can be prepared in the laboratory through the use of deuterated acetic anhydride. The utility of the method was assessed for the analysis of ACTH and insulin in samples from horses suffering from pituitary pars intermedia dysfunction (PPID).<\/jats:p>","DOI":"10.1002\/dta.3762","type":"journal-article","created":{"date-parts":[[2024,7,9]],"date-time":"2024-07-09T00:16:46Z","timestamp":1720484206000},"page":"589-600","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The detection of ACTH and insulin in equine plasma by solid\u2010phase extraction and micro\u2010flow LC\/MSMS"],"prefix":"10.1002","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0423-6120","authenticated-orcid":false,"given":"Rohan","family":"Steel","sequence":"first","affiliation":[{"name":"Biological Research Unit Racing Analytical Services Ltd  Flemington Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Timms","sequence":"additional","affiliation":[{"name":"Biological Research Unit Racing Analytical Services Ltd  Flemington Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7675-9126","authenticated-orcid":false,"given":"Nicholas","family":"Bamford","sequence":"additional","affiliation":[{"name":"Veterinary Biosciences Melbourne University  Parkville Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5731-6602","authenticated-orcid":false,"given":"Robert","family":"Spence","sequence":"additional","affiliation":[{"name":"Department of Biological and Environmental Sciences Queensland University of Technology  Brisbane Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Sillence","sequence":"additional","affiliation":[{"name":"Department of Biological and Environmental Sciences Queensland University of Technology  Brisbane Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,7,8]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00421\u2010008\u20100802\u2010y"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjp.2008.99"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2010.12.052"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2008.04.060"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216\u2010010\u20104565\u2010z"},{"key":"e_1_2_5_7_1","first-page":"182","volume-title":"Proceedings of the 20th International Conference of Racing Analysts and Veterinarians","author":"Taylor P","year":"2014"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.2765"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/hvae034"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/ansa.202200027"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci9100556"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2013.00143"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/evj.13925"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.0c04568"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20101\u20100716\u20102978\u20109_28"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2004.03.038"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2005.02.090"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.1624"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijms.2011.08.005"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20101\u201060761\u2010157\u20108_7"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.2c00405"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1080\/14789450.2022.2134780"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.3544"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.2880"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.4155\/bio.13.281"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.1585"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.2671"}],"container-title":["Drug Testing and Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/am-pdf\/10.1002\/dta.3762","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dta.3762","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T22:03:41Z","timestamp":1760393021000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/dta.3762"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,8]]},"references-count":27,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,5]]}},"alternative-id":["10.1002\/dta.3762"],"URL":"https:\/\/doi.org\/10.1002\/dta.3762","archive":["Portico"],"relation":{},"ISSN":["1942-7603","1942-7611"],"issn-type":[{"value":"1942-7603","type":"print"},{"value":"1942-7611","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,8]]},"assertion":[{"value":"2024-04-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}