{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T20:13:23Z","timestamp":1787170403634,"version":"build-2736575974"},"reference-count":41,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,8,16]],"date-time":"2024-08-16T00:00:00Z","timestamp":1723766400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012510","name":"Kulturministeriet","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012510","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017190","name":"Anti Doping Danmark","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100017190","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Drug Testing and Analysis"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Salbutamol is a common short\u2010acting beta\n                    <jats:sub>2<\/jats:sub>\n                    \u2010adrenergic agonist used in treatment of asthma and exercise\u2010induced bronchoconstriction but also possesses anabolic and metabolic actions in skeletal muscle. As a chiral compound, salbutamol is a racemic 1:1 mixture of two enantiomers, (R)\u2010salbutamol and (S)\u2010salbutamol, which exhibit divergent pharmacokinetic and pharmacodynamic actions. Despite salbutamol being available for decades, information on the enantioselective disposition of salbutamol enantiomers in human skeletal muscle is absent. In this study, we determined concentrations of (R)\u2010salbutamol and (S)\u2010salbutamol by UHPLC\u2013MS\/MS in arterial plasma and\n                    <jats:italic>vastus lateralis<\/jats:italic>\n                    muscle samples from 12 lean young men 2\u00bd and 7\u2009h following ingestion of 24\u2009mg oral salbutamol. Mean (range) arterial plasma concentrations were 10\u2010fold higher (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001) for (S)\u2010salbutamol than (R)\u2010salbutamol, being 33(9\u201362) and 49(30\u201384) ng\u00b7mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    for (S)\u2010salbutamol and 4 (1\u20106) and 4 (2\u20105) ng\u00b7mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    for (R)\u2010salbutamol 2\u00bd and 7\u2009h following administration, respectively, reflecting faster elimination of the (R)\u2010enantiomer. Mean (range) muscle concentrations were higher (p\u2009&lt;\u20090.001) for (S)\u2010salbutamol than (R)\u2010salbutamol 2\u00bd\u00a0h (0.17 [0.1\u20130.26] vs. 0.04 [0.02\u20130.06]) and 7\u2009h (0.31 [0.21\u20130.46] vs. 0.06 [0.04\u20130.12] ng\u00b7mg\n                    <jats:sub>d.w.<\/jats:sub>\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) after administration. However, muscle:plasma partition coefficient was two\u2010fold higher (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001) for (R)\u2010salbutamol than (S)\u2010salbutamol 7\u2009h following administration. These observations demonstrate that oral salbutamol exhibits enantioselective disposition in systemic circulation and muscle favoring the (S)\u2010enantiomer but with higher relative partitioning of the (R)\u2010enantiomer in skeletal muscle. Furthermore, the concentration\u2010time profiles of salbutamol enantiomers are different in skeletal muscle and systemic circulation following oral ingestion. These findings have implications for the application of chiral switch (R)\u2010salbutamol in doping control.\n                  <\/jats:p>","DOI":"10.1002\/dta.3787","type":"journal-article","created":{"date-parts":[[2024,8,16]],"date-time":"2024-08-16T03:04:47Z","timestamp":1723777487000},"page":"842-849","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Beta\n                    <sub>2<\/sub>\n                    \u2010adrenergic agonist salbutamol exhibits enantioselective disposition in skeletal muscle of lean young men following oral administration"],"prefix":"10.1002","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6201-2483","authenticated-orcid":false,"given":"Morten","family":"Hostrup","sequence":"first","affiliation":[{"name":"August Krogh Section for Human Physiology, Department of Nutrition, Exercise and Sports University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3409-8769","authenticated-orcid":false,"given":"Glenn A.","family":"Jacobson","sequence":"additional","affiliation":[{"name":"School of Pharmacy and Pharmacology, College of Health and Medicine University of Tasmania  Hobart Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kasper","family":"Eibye","sequence":"additional","affiliation":[{"name":"August Krogh Section for Human Physiology, Department of Nutrition, Exercise and Sports University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian K.","family":"Narkowicz","sequence":"additional","affiliation":[{"name":"School of Pharmacy and Pharmacology, College of Health and Medicine University of Tasmania  Hobart Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David S.","family":"Nichols","sequence":"additional","affiliation":[{"name":"Central Science Laboratory University of Tasmania  Hobart Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7461-1264","authenticated-orcid":false,"given":"S\u00f8ren","family":"Jessen","sequence":"additional","affiliation":[{"name":"August Krogh Section for Human Physiology, Department of Nutrition, Exercise and Sports University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,8,16]]},"reference":[{"key":"e_1_2_7_2_1","unstructured":"World Anti\u2010Doping Agency.Minutes of the WADA Executive Committee Meeting 20 September 2018.https:\/\/www.wada-ama.org\/sites\/default\/files\/resources\/files\/executive_committee_meeting_minutes_20092018.pdf. 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